Tuesday, July 17, 2007
Tuesday, July 10, 2007
The First Big Hitter In Genetic Change: Androgens
Make no mistake; nothing else mentioned in this book will help you grow like androgens, as they are the basic building block for increasing muscle size and strength. All other things only serve to enhance androgens. So whether you use a natural testosterone booster or a pro-hormone or real juice, you need the basics to build your genetic house. Androgens have been shown to create new muscle growth in multiple ways, both by increasing protein synthesis (filling up your muscle balloons) and increasing the differentiation of satellite cells (making more muscle balloons). That makes them the ultimate starting point for a genetic anabolic cocktail.
What are androgens? Quite simply they are testosterone and its derivatives, both legal and illegal. Guess when your body produces the most testosterone? You got it, puberty! That’s why you can gain mad muscle cells if you simply work out and eat right when you are in high school. Eating right is important because your body isn’t going to make new muscle cells if it doesn’t have the protein to fill up the ones it already has (which is why so many high school students work out without results). Growing muscle during puberty happens in the kitchen, not just the gym, but that is another book.
Androgens are the most potent muscle builders you can take. Luckily with our products you get the best of both worlds, increased androgen production without the negative side effects that SCARE the hell out of most people. You can start small and use something like Formadrol Extreme or you can jump up to our prohormones (Liquid Masterdrol and Methyl 1-D) or you can go the illegal route and hit juice (we don’t recommend this by the way). People doing our Trifecta Stack are routinely hitting 8-12lbs of muscle in a month without losing their gains. If you juice, you can probably increase that to 15-17lbs but when you grow that quickly, you rarely keep your gains. Slow, steady gains might not feel as exciting, but they are the way to build a body, not an ego! The combination of Stanolone (Liquid Masterdrol) plus Testosterone (Methyl 1-D) is a very powerful combination and gives you the ULTIMATE in safety and also gives REAL gains that you would see from a SANE steroid cycle, without the negatives! All these products are available from LG Sciences on the web at www.lgsciences.com
So, how do you get a spike in androgens? Many ways… The first way is to use an herbal product that typically will trick your body into increasing output of Luteinizing Hormone. These were made popular with products like Tribulusä and have come a long way with the advent of newer ingredients like Long Jackä and others out on the market. Some of them, like Horny Goat Weedä only serve to boost sex drive and have not been shown to enhance testosterone production.
Another way is to use a natural testosterone booster like LG Sciences Formadrol Extreme, which tricks your body into producing more testosterone by blocking estrogen in the body. This can give you levels above even the very highest natural range (a normal man ranges from 250 ng/dl of testosterone to 900 ng/dl). Formadrol Extreme and similar products trick your body into producing more testosterone and can be very useful as a natural anabolic booster, getting your testosterone levels into the 1400ng/dl range. It is also great for Post Cycle Therapy after a steroid or pro-hormone cycle. They are also useful in combating gynecomastia, commonly called "bitch tits".
The third way to boost your androgen levels is the use of pro-hormones. First made popular by "Andro", pro-hormones use the basic building blocks of testosterone to force your body into producing more testosterone through the action of enzymes. The first generation of testosterone boosters, like Andro, had tons of side effects and didn’t work very well. Newer pro-hormones like 1-AD were better, but were still not up to the full value of a steroid cycle. The latest crop of pro-hormones, like Methyl-1-D and Liquid Masterdrol by LG Sciences, reduce the side effects, prolong the half-life in the body and reduce conversion to unwanted, side effect producing by-products like Estrogen. The use of pro-hormones can give you testosterone levels that rival even the strongest steroids, raising your testosterone levels to 3000-3500 ng/dl if used properly. All products are available from www.lgsciences.com
LG Sciences is a pro-hormone that is formulated to reduce side effects, provide maximum safety and give you AMAZING results. It’s the real basis for our genetics-changing stack. For a more advanced stack, use Liquid-Masterdrol along with Methyl-1-D for the best possible combination of prohormones that hit both the more androgenic (Liquid Masterdrol) and more anabolic (Methyl 1-D) aspects of growth. Methyl 1-D is available from www.lgsciences.com
PROHORMONES SHOULD NEVER BE USED BY ANYONE UNDER 21
The fourth way to boost androgen levels is to use artificial illegal steroids to boost your testosterone levels above even the highest legal levels that pro-hormones can give. I am not here to make a value statement about steroids, but I am just pointing out the option to anyone that is in a foreign country that allows steroid use or is using androgens with a doctor’s prescription.
We highly suggest that you do not use illegal anabolic steroids for obvious reasons and we hope that you respect the law and your body by using natural substances that are safer alternatives. If you do use steroids however, we recommend that you do so safely, cycling off them for at least a month at a time, taking supplements like Fish Oil to cleanse your liver and reduce the chance of cardiovascular side effects. Now, that being said, steroids are obviously not that dangerous, since prescription compounds, such as Testosterone Gel, are being pushed as the next great development (never mind that the Testosterone Gels on the market are loaded with side effects including conversion to DHT which aggravates the prostate and estrogen that can promote breast cancer, even in men). Once the root of all evil, steroids are suddenly completely safe and side effect free when turned into a prescription drug. Wake up people! Again, we don’t encourage you to use steroids, but if you do, please consider doing them safely (there is a section at the end of good supplements to take with steroids that will offer some nice protection from their side effects).
So, if you are going to do a cycle of steroids, this is the perfect time to add in some serious genetic expression supplements to maximize your growth. One of the funniest things I see is the juice crowd bashing the supplement crowd, when in reality someone on a cycle will probably benefit the most from proper supplementation.
Studies show that androgens do indeed cause genetic changes that can benefit powerlifters and bodybuilders.
Here you see how increasing testosterone through natural boosting, pro-hormones or illegal anabolic steroids can vastly increase satellite muscle cell expression. This is the first real pathway that the body uses to increase muscle size and the number of muscle cells. By increasing the raw number of satellite cells, testosterone is the foundation of our genetic altering arsenal.
Skeletal muscle morphology in power-lifters with and without anabolic steroids.Eriksson A, Kadi F, Malm C, Thornell LE.Department of Integrative Medical Biology, Section for Anatomy, Umea
University, 901 87 Umea, Sweden.
The morphological appearance of the vastus lateralis (VL) muscle from high-level power-lifters on long-term anabolic steroid supplementation (PAS) and power-lifters never taking anabolic steroids (P) was compared. The effects of long- and short-term supplementation were compared. Enzyme-immunohistochemical investigations were performed to assess muscle fiber type composition, fiber area, and number of myonuclei per fiber, internal myonuclei, myonuclear domains and proportion of satellite cells. The PAS group had larger type I, IIA, IIAB and IIC fiber areas (p<0.05).> or = 5 myonuclei. The results of AS on VL morphology in this study were similar to previously reported short-term effects of AS on VL. The initial effects from AS appear to be maintained for several years.
Androgen receptor in human skeletal muscle and cultured muscle satellite cells: up-regulation by androgen treatment.
Sinha-Hikim I, Taylor WE, Gonzalez-Cadavid NF, Zheng W, Bhasin S.Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA.
Androgens stimulate myogenesis, but we do not know what cell types within human skeletal muscle express the androgen receptor (AR) protein and are the target of androgen action. Because testosterone promotes the commitment of pluripotent, mesenchymal cells into myogenic lineage, we hypothesized that AR would be expressed in mesenchymal precursor cells in the skeletal muscle. AR expression was evaluated by immunohistochemical staining, confocal immunofluorescence, and immunoelectron microscopy in sections of vastus lateralis from healthy men before and after treatment with a supraphysiological dose of testosterone enanthate. Satellite cell cultures from human skeletal muscle were also tested for AR expression. AR protein was expressed predominantly in satellite cells, identified by their location outside sarcolemma and inside basal lamina, and by CD34 and C-met staining. Many myonuclei in muscle fibers also demonstrated AR immunostaining. Additionally, CD34+ stem cells in the interstitium, fibroblasts, and mast cells expressed AR immunoreactivity. AR expression was also observed in vascular endothelial and smooth muscle cells. Immunoelectron microscopy revealed aggregation of immunogold particles in nucleoli of satellite cells and myonuclei; testosterone treatment increased nucleolar AR density. In enriched cultures of human satellite cells, more than 95% of cells stained for CD34 and C-met, confirming their identity as satellite cells, and expressed AR protein. AR mRNA and protein expression in satellite cell cultures was confirmed by RT-PCR, reverse transcription and real-time PCR, sequencing of RT-PCR product, and Western blot analysis. Incubation of satellite cell cultures with supraphysiological testosterone and dihydrotestosterone concentrations (100 nm testosterone and 30 nm dihydrotestosterone) modestly increased AR protein levels. We conclude that AR is expressed in several cell types in human skeletal muscle, including satellite cells, fibroblasts, CD34+ precursor cells, vascular endothelial, smooth muscle cells, and mast cells. Satellite cells are the predominant site of AR expression. These observations support the hypothesis that androgens increase muscle mass in part by acting on several cell types to regulate the differentiation of mesenchymal precursor cells in the skeletal muscle.
That being said, we can safely increase testosterone using supplements and don’t need illegal anabolic steroids! Pro-hormones boost natural testosterone by giving you the building blocks for testosterone and other steroid hormones. Pro-hormones are the safest, most effective way to boost testosterone without using drugs. The second way is to use natural testosterone boosters. There are many on the market, like tribulus, long jack and others. The best combination is an anti-estrogen that tricks your body into producing more testosterone. There are many of these products on the market, but the latest generations are far more effective and have fewer or no negative effects on the body. Here is what some peer reviewed journal entries say about using testosterone to activate satellite cells:
Testosterone-induced muscle hypertrophy is associated with an increase in satellite cell number in healthy, young men.
Sinha-Hikim I, Roth SM, Lee MI, Bhasin S.Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA.
Testosterone (T) supplementation in men induces muscle fiber hypertrophy. We hypothesized that T-induced increase in muscle fiber size is associated with a dose-dependent increase in satellite cell number. We quantitated satellite cell and myonuclear number by using direct counting and spatial orientation methods in biopsies of vastus lateralis obtained at baseline and after 20 wk of treatment with a gonadotropin-releasing hormone agonist and a 125-, 300-, or 600-mg weekly dose of T enanthate. T administration was associated with a significant increase in myonuclear number in men receiving 300- and 600-mg doses. The posttreatment percent satellite cell number, obtained by direct counting, differed significantly among the three groups (ANCOVA P < 0.000001); the mean posttreatment values (5.0 and 15.0%) in men treated with 300- and 600-mg doses were greater than baseline (2.5 and 2.5%, respectively, P < 0.05 vs. baseline). The absolute satellite cell number measured by spatial orientation at 20 wk (1.5 and 4.0/mm) was significantly greater than baseline (0.3 and 0.6/mm) in men receiving the 300- and 600-mg doses (P < 0.05). The change in percent satellite cell number correlated with changes in total (r = 0.548) and free T concentrations (r = 0.468). Satellite cell and mitochondrial areas were significantly higher and the nuclear-to-cytoplasmic ratio lower after treatment with 300- and 600-mg doses. We conclude that T-induced muscle fiber hypertrophy is associated with an increase in satellite cell number, a proportionate increase in myonuclear number, and changes in satellite cell ultrastructure.
Androgen regulation of satellite cell function.
Chen Y, Zajac JD, MacLean HE.Department of Medicine, University of Melbourne, Austin Health, Heidelberg, Victoria 3084, Australia.
Androgen treatment can enhance the size and strength of muscle. However, the mechanisms of androgen action in skeletal muscle are poorly understood. This review discusses potential mechanisms by which androgens regulate satellite cell activation and function. Studies have demonstrated that androgen administration increases satellite cell numbers in animals and humans in a dose-dependent manner. Moreover, androgens increase androgen receptor levels in satellite cells. In vitro, the results are contradictory as to whether androgens regulate satellite cell proliferation or differentiation. IGF-I is one major target of androgen action in satellite cells. In addition, the possibility of non-genomic actions of androgens on satellite cells is discussed. In summary, this review focuses on exploring potential mechanisms through which androgens regulate satellite cells, by analyzing developments from research in this area.
Androgen receptor in human skeletal muscle and cultured muscle satellite cells: up-regulation by androgen treatment.
Sinha-Hikim I, Taylor WE, Gonzalez-Cadavid NF, Zheng W, Bhasin S.Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA.
Androgens stimulate myogenesis, but we do not know what cell types within human skeletal muscle express the androgen receptor (AR) protein and are the target of androgen action. Because testosterone promotes the commitment of pluripotent, mesenchymal cells into myogenic lineage, we hypothesized that AR would be expressed in mesenchymal precursor cells in the skeletal muscle. AR expression was evaluated by immunohistochemical staining, confocal immunofluorescence, and immunoelectron microscopy in sections of vastus lateralis from healthy men before and after treatment with a supraphysiological dose of testosterone enanthate. Satellite cell cultures from human skeletal muscle were also tested for AR expression. AR protein was expressed predominantly in satellite cells, identified by their location outside sarcolemma and inside basal lamina, and by CD34 and C-met staining. Many myonuclei in muscle fibers also demonstrated AR immunostaining. Additionally, CD34+ stem cells in the interstitium, fibroblasts, and mast cells expressed AR immunoreactivity. AR expression was also observed in vascular endothelial and smooth muscle cells. Immunoelectron microscopy revealed aggregation of immunogold particles in nucleoli of satellite cells and myonuclei; testosterone treatment increased nucleolar AR density. In enriched cultures of human satellite cells, more than 95% of cells stained for CD34 and C-met, confirming their identity as satellite cells, and expressed AR protein. AR mRNA and protein expression in satellite cell cultures was confirmed by RT-PCR, reverse transcription and real-time PCR, sequencing of RT-PCR product, and Western blot analysis. Incubation of satellite cell cultures with supraphysiological testosterone and dihydrotestosterone concentrations (100 nm testosterone and 30 nm dihydrotestosterone) modestly increased AR protein levels. We conclude that AR is expressed in several cell types in human skeletal muscle, including satellite cells, fibroblasts, CD34+ precursor cells, vascular endothelial, smooth muscle cells, and mast cells. Satellite cells are the predominant site of AR expression. These observations support the hypothesis that androgens increase muscle mass in part by acting on several cell types to regulate the differentiation of mesenchymal precursor cells in the skeletal muscle.
Testosterone action on skeletal muscle.
Herbst KL, Bhasin S.UCLA School of Medicine, Charles R. Drew University, Los Angeles, California 90059, USA.
PURPOSE OF REVIEW: To highlight recent data demonstrating direct anabolic effects of androgens on the mammalian skeletal muscle and review the mechanisms by which testosterone regulates body composition. RECENT FINDINGS: Testosterone increases lean body mass and decreases fat mass in young men; the magnitude of the changes induced by testosterone in lean and fat mass are correlated with testosterone dose and the prevalent testosterone concentrations. Older men are as responsive to the anabolic effects of testosterone on the muscle as young men, but have increased frequency of adverse events with higher testosterone doses. This reciprocal change in lean and fat mass induced by androgens is best explained by the hypothesis that androgens promote the commitment of mesenchymal pluripotent cells into myogenic lineage and inhibit adipogenesis through an androgen receptor mediated pathway. Resident muscle satellite cells increase in number with testosterone administration forming myoblasts leading to greater numbers of myonuclei in larger myofibers. Testosterone administration is associated with increased size of motor neurons. The roles of 5-alpha reduction and aromatization of testosterone into dihydrotestosterone and estradiol, respectively, in mediating testosterone effects on body composition are poorly understood. SUMMARY: Testosterone induces skeletal muscle hypertrophy by multiple mechanisms, including its effects in modulating the commitment of pluripotent mesenchymal cells. These changes in skeletal muscle lead to improved muscle strength and leg power; however, further studies are needed to determine the effects of testosterone on physical function and health-related outcomes in sarcopenia associated with aging and chronic illness.
Here you see how increasing testosterone through natural boosting, pro-hormones or illegal anabolic steroids can vastly increase satellite muscle cell expression. This is the first real pathway that the body uses to increase muscle size and the number of muscle cells. By increasing the raw number of satellite cells, testosterone is the foundation of our genetic altering arsenal.
Eric D. Marchewitz, is one of the leading supplement experts in the country, his articles online are taken from his best selling book " Supplements For Genetic Growth " which explains how you can increase the number of muscle cells in your body using supplement stacks available at any health food store. This book will demonstrate how the body will try and resist your efforts to grow insane muscles and how, as you age, the ability to create new muscle cells decline so make those cells now! The book is backed by science! This is the most amazing break through in supplement history! The book is available from the LG Sciences website www.lgsciences.com
Make no mistake; nothing else mentioned in this book will help you grow like androgens, as they are the basic building block for increasing muscle size and strength. All other things only serve to enhance androgens. So whether you use a natural testosterone booster or a pro-hormone or real juice, you need the basics to build your genetic house. Androgens have been shown to create new muscle growth in multiple ways, both by increasing protein synthesis (filling up your muscle balloons) and increasing the differentiation of satellite cells (making more muscle balloons). That makes them the ultimate starting point for a genetic anabolic cocktail.
What are androgens? Quite simply they are testosterone and its derivatives, both legal and illegal. Guess when your body produces the most testosterone? You got it, puberty! That’s why you can gain mad muscle cells if you simply work out and eat right when you are in high school. Eating right is important because your body isn’t going to make new muscle cells if it doesn’t have the protein to fill up the ones it already has (which is why so many high school students work out without results). Growing muscle during puberty happens in the kitchen, not just the gym, but that is another book.
Androgens are the most potent muscle builders you can take. Luckily with our products you get the best of both worlds, increased androgen production without the negative side effects that SCARE the hell out of most people. You can start small and use something like Formadrol Extreme or you can jump up to our prohormones (Liquid Masterdrol and Methyl 1-D) or you can go the illegal route and hit juice (we don’t recommend this by the way). People doing our Trifecta Stack are routinely hitting 8-12lbs of muscle in a month without losing their gains. If you juice, you can probably increase that to 15-17lbs but when you grow that quickly, you rarely keep your gains. Slow, steady gains might not feel as exciting, but they are the way to build a body, not an ego! The combination of Stanolone (Liquid Masterdrol) plus Testosterone (Methyl 1-D) is a very powerful combination and gives you the ULTIMATE in safety and also gives REAL gains that you would see from a SANE steroid cycle, without the negatives! All these products are available from LG Sciences on the web at www.lgsciences.com
So, how do you get a spike in androgens? Many ways… The first way is to use an herbal product that typically will trick your body into increasing output of Luteinizing Hormone. These were made popular with products like Tribulusä and have come a long way with the advent of newer ingredients like Long Jackä and others out on the market. Some of them, like Horny Goat Weedä only serve to boost sex drive and have not been shown to enhance testosterone production.
Another way is to use a natural testosterone booster like LG Sciences Formadrol Extreme, which tricks your body into producing more testosterone by blocking estrogen in the body. This can give you levels above even the very highest natural range (a normal man ranges from 250 ng/dl of testosterone to 900 ng/dl). Formadrol Extreme and similar products trick your body into producing more testosterone and can be very useful as a natural anabolic booster, getting your testosterone levels into the 1400ng/dl range. It is also great for Post Cycle Therapy after a steroid or pro-hormone cycle. They are also useful in combating gynecomastia, commonly called "bitch tits".
The third way to boost your androgen levels is the use of pro-hormones. First made popular by "Andro", pro-hormones use the basic building blocks of testosterone to force your body into producing more testosterone through the action of enzymes. The first generation of testosterone boosters, like Andro, had tons of side effects and didn’t work very well. Newer pro-hormones like 1-AD were better, but were still not up to the full value of a steroid cycle. The latest crop of pro-hormones, like Methyl-1-D and Liquid Masterdrol by LG Sciences, reduce the side effects, prolong the half-life in the body and reduce conversion to unwanted, side effect producing by-products like Estrogen. The use of pro-hormones can give you testosterone levels that rival even the strongest steroids, raising your testosterone levels to 3000-3500 ng/dl if used properly. All products are available from www.lgsciences.com
LG Sciences is a pro-hormone that is formulated to reduce side effects, provide maximum safety and give you AMAZING results. It’s the real basis for our genetics-changing stack. For a more advanced stack, use Liquid-Masterdrol along with Methyl-1-D for the best possible combination of prohormones that hit both the more androgenic (Liquid Masterdrol) and more anabolic (Methyl 1-D) aspects of growth. Methyl 1-D is available from www.lgsciences.com
PROHORMONES SHOULD NEVER BE USED BY ANYONE UNDER 21
The fourth way to boost androgen levels is to use artificial illegal steroids to boost your testosterone levels above even the highest legal levels that pro-hormones can give. I am not here to make a value statement about steroids, but I am just pointing out the option to anyone that is in a foreign country that allows steroid use or is using androgens with a doctor’s prescription.
We highly suggest that you do not use illegal anabolic steroids for obvious reasons and we hope that you respect the law and your body by using natural substances that are safer alternatives. If you do use steroids however, we recommend that you do so safely, cycling off them for at least a month at a time, taking supplements like Fish Oil to cleanse your liver and reduce the chance of cardiovascular side effects. Now, that being said, steroids are obviously not that dangerous, since prescription compounds, such as Testosterone Gel, are being pushed as the next great development (never mind that the Testosterone Gels on the market are loaded with side effects including conversion to DHT which aggravates the prostate and estrogen that can promote breast cancer, even in men). Once the root of all evil, steroids are suddenly completely safe and side effect free when turned into a prescription drug. Wake up people! Again, we don’t encourage you to use steroids, but if you do, please consider doing them safely (there is a section at the end of good supplements to take with steroids that will offer some nice protection from their side effects).
So, if you are going to do a cycle of steroids, this is the perfect time to add in some serious genetic expression supplements to maximize your growth. One of the funniest things I see is the juice crowd bashing the supplement crowd, when in reality someone on a cycle will probably benefit the most from proper supplementation.
Studies show that androgens do indeed cause genetic changes that can benefit powerlifters and bodybuilders.
Here you see how increasing testosterone through natural boosting, pro-hormones or illegal anabolic steroids can vastly increase satellite muscle cell expression. This is the first real pathway that the body uses to increase muscle size and the number of muscle cells. By increasing the raw number of satellite cells, testosterone is the foundation of our genetic altering arsenal.
Skeletal muscle morphology in power-lifters with and without anabolic steroids.Eriksson A, Kadi F, Malm C, Thornell LE.Department of Integrative Medical Biology, Section for Anatomy, Umea
University, 901 87 Umea, Sweden.
The morphological appearance of the vastus lateralis (VL) muscle from high-level power-lifters on long-term anabolic steroid supplementation (PAS) and power-lifters never taking anabolic steroids (P) was compared. The effects of long- and short-term supplementation were compared. Enzyme-immunohistochemical investigations were performed to assess muscle fiber type composition, fiber area, and number of myonuclei per fiber, internal myonuclei, myonuclear domains and proportion of satellite cells. The PAS group had larger type I, IIA, IIAB and IIC fiber areas (p<0.05).> or = 5 myonuclei. The results of AS on VL morphology in this study were similar to previously reported short-term effects of AS on VL. The initial effects from AS appear to be maintained for several years.
Androgen receptor in human skeletal muscle and cultured muscle satellite cells: up-regulation by androgen treatment.
Sinha-Hikim I, Taylor WE, Gonzalez-Cadavid NF, Zheng W, Bhasin S.Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA.
Androgens stimulate myogenesis, but we do not know what cell types within human skeletal muscle express the androgen receptor (AR) protein and are the target of androgen action. Because testosterone promotes the commitment of pluripotent, mesenchymal cells into myogenic lineage, we hypothesized that AR would be expressed in mesenchymal precursor cells in the skeletal muscle. AR expression was evaluated by immunohistochemical staining, confocal immunofluorescence, and immunoelectron microscopy in sections of vastus lateralis from healthy men before and after treatment with a supraphysiological dose of testosterone enanthate. Satellite cell cultures from human skeletal muscle were also tested for AR expression. AR protein was expressed predominantly in satellite cells, identified by their location outside sarcolemma and inside basal lamina, and by CD34 and C-met staining. Many myonuclei in muscle fibers also demonstrated AR immunostaining. Additionally, CD34+ stem cells in the interstitium, fibroblasts, and mast cells expressed AR immunoreactivity. AR expression was also observed in vascular endothelial and smooth muscle cells. Immunoelectron microscopy revealed aggregation of immunogold particles in nucleoli of satellite cells and myonuclei; testosterone treatment increased nucleolar AR density. In enriched cultures of human satellite cells, more than 95% of cells stained for CD34 and C-met, confirming their identity as satellite cells, and expressed AR protein. AR mRNA and protein expression in satellite cell cultures was confirmed by RT-PCR, reverse transcription and real-time PCR, sequencing of RT-PCR product, and Western blot analysis. Incubation of satellite cell cultures with supraphysiological testosterone and dihydrotestosterone concentrations (100 nm testosterone and 30 nm dihydrotestosterone) modestly increased AR protein levels. We conclude that AR is expressed in several cell types in human skeletal muscle, including satellite cells, fibroblasts, CD34+ precursor cells, vascular endothelial, smooth muscle cells, and mast cells. Satellite cells are the predominant site of AR expression. These observations support the hypothesis that androgens increase muscle mass in part by acting on several cell types to regulate the differentiation of mesenchymal precursor cells in the skeletal muscle.
That being said, we can safely increase testosterone using supplements and don’t need illegal anabolic steroids! Pro-hormones boost natural testosterone by giving you the building blocks for testosterone and other steroid hormones. Pro-hormones are the safest, most effective way to boost testosterone without using drugs. The second way is to use natural testosterone boosters. There are many on the market, like tribulus, long jack and others. The best combination is an anti-estrogen that tricks your body into producing more testosterone. There are many of these products on the market, but the latest generations are far more effective and have fewer or no negative effects on the body. Here is what some peer reviewed journal entries say about using testosterone to activate satellite cells:
Testosterone-induced muscle hypertrophy is associated with an increase in satellite cell number in healthy, young men.
Sinha-Hikim I, Roth SM, Lee MI, Bhasin S.Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA.
Testosterone (T) supplementation in men induces muscle fiber hypertrophy. We hypothesized that T-induced increase in muscle fiber size is associated with a dose-dependent increase in satellite cell number. We quantitated satellite cell and myonuclear number by using direct counting and spatial orientation methods in biopsies of vastus lateralis obtained at baseline and after 20 wk of treatment with a gonadotropin-releasing hormone agonist and a 125-, 300-, or 600-mg weekly dose of T enanthate. T administration was associated with a significant increase in myonuclear number in men receiving 300- and 600-mg doses. The posttreatment percent satellite cell number, obtained by direct counting, differed significantly among the three groups (ANCOVA P < 0.000001); the mean posttreatment values (5.0 and 15.0%) in men treated with 300- and 600-mg doses were greater than baseline (2.5 and 2.5%, respectively, P < 0.05 vs. baseline). The absolute satellite cell number measured by spatial orientation at 20 wk (1.5 and 4.0/mm) was significantly greater than baseline (0.3 and 0.6/mm) in men receiving the 300- and 600-mg doses (P < 0.05). The change in percent satellite cell number correlated with changes in total (r = 0.548) and free T concentrations (r = 0.468). Satellite cell and mitochondrial areas were significantly higher and the nuclear-to-cytoplasmic ratio lower after treatment with 300- and 600-mg doses. We conclude that T-induced muscle fiber hypertrophy is associated with an increase in satellite cell number, a proportionate increase in myonuclear number, and changes in satellite cell ultrastructure.
Androgen regulation of satellite cell function.
Chen Y, Zajac JD, MacLean HE.Department of Medicine, University of Melbourne, Austin Health, Heidelberg, Victoria 3084, Australia.
Androgen treatment can enhance the size and strength of muscle. However, the mechanisms of androgen action in skeletal muscle are poorly understood. This review discusses potential mechanisms by which androgens regulate satellite cell activation and function. Studies have demonstrated that androgen administration increases satellite cell numbers in animals and humans in a dose-dependent manner. Moreover, androgens increase androgen receptor levels in satellite cells. In vitro, the results are contradictory as to whether androgens regulate satellite cell proliferation or differentiation. IGF-I is one major target of androgen action in satellite cells. In addition, the possibility of non-genomic actions of androgens on satellite cells is discussed. In summary, this review focuses on exploring potential mechanisms through which androgens regulate satellite cells, by analyzing developments from research in this area.
Androgen receptor in human skeletal muscle and cultured muscle satellite cells: up-regulation by androgen treatment.
Sinha-Hikim I, Taylor WE, Gonzalez-Cadavid NF, Zheng W, Bhasin S.Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA.
Androgens stimulate myogenesis, but we do not know what cell types within human skeletal muscle express the androgen receptor (AR) protein and are the target of androgen action. Because testosterone promotes the commitment of pluripotent, mesenchymal cells into myogenic lineage, we hypothesized that AR would be expressed in mesenchymal precursor cells in the skeletal muscle. AR expression was evaluated by immunohistochemical staining, confocal immunofluorescence, and immunoelectron microscopy in sections of vastus lateralis from healthy men before and after treatment with a supraphysiological dose of testosterone enanthate. Satellite cell cultures from human skeletal muscle were also tested for AR expression. AR protein was expressed predominantly in satellite cells, identified by their location outside sarcolemma and inside basal lamina, and by CD34 and C-met staining. Many myonuclei in muscle fibers also demonstrated AR immunostaining. Additionally, CD34+ stem cells in the interstitium, fibroblasts, and mast cells expressed AR immunoreactivity. AR expression was also observed in vascular endothelial and smooth muscle cells. Immunoelectron microscopy revealed aggregation of immunogold particles in nucleoli of satellite cells and myonuclei; testosterone treatment increased nucleolar AR density. In enriched cultures of human satellite cells, more than 95% of cells stained for CD34 and C-met, confirming their identity as satellite cells, and expressed AR protein. AR mRNA and protein expression in satellite cell cultures was confirmed by RT-PCR, reverse transcription and real-time PCR, sequencing of RT-PCR product, and Western blot analysis. Incubation of satellite cell cultures with supraphysiological testosterone and dihydrotestosterone concentrations (100 nm testosterone and 30 nm dihydrotestosterone) modestly increased AR protein levels. We conclude that AR is expressed in several cell types in human skeletal muscle, including satellite cells, fibroblasts, CD34+ precursor cells, vascular endothelial, smooth muscle cells, and mast cells. Satellite cells are the predominant site of AR expression. These observations support the hypothesis that androgens increase muscle mass in part by acting on several cell types to regulate the differentiation of mesenchymal precursor cells in the skeletal muscle.
Testosterone action on skeletal muscle.
Herbst KL, Bhasin S.UCLA School of Medicine, Charles R. Drew University, Los Angeles, California 90059, USA.
PURPOSE OF REVIEW: To highlight recent data demonstrating direct anabolic effects of androgens on the mammalian skeletal muscle and review the mechanisms by which testosterone regulates body composition. RECENT FINDINGS: Testosterone increases lean body mass and decreases fat mass in young men; the magnitude of the changes induced by testosterone in lean and fat mass are correlated with testosterone dose and the prevalent testosterone concentrations. Older men are as responsive to the anabolic effects of testosterone on the muscle as young men, but have increased frequency of adverse events with higher testosterone doses. This reciprocal change in lean and fat mass induced by androgens is best explained by the hypothesis that androgens promote the commitment of mesenchymal pluripotent cells into myogenic lineage and inhibit adipogenesis through an androgen receptor mediated pathway. Resident muscle satellite cells increase in number with testosterone administration forming myoblasts leading to greater numbers of myonuclei in larger myofibers. Testosterone administration is associated with increased size of motor neurons. The roles of 5-alpha reduction and aromatization of testosterone into dihydrotestosterone and estradiol, respectively, in mediating testosterone effects on body composition are poorly understood. SUMMARY: Testosterone induces skeletal muscle hypertrophy by multiple mechanisms, including its effects in modulating the commitment of pluripotent mesenchymal cells. These changes in skeletal muscle lead to improved muscle strength and leg power; however, further studies are needed to determine the effects of testosterone on physical function and health-related outcomes in sarcopenia associated with aging and chronic illness.
Here you see how increasing testosterone through natural boosting, pro-hormones or illegal anabolic steroids can vastly increase satellite muscle cell expression. This is the first real pathway that the body uses to increase muscle size and the number of muscle cells. By increasing the raw number of satellite cells, testosterone is the foundation of our genetic altering arsenal.
Eric D. Marchewitz, is one of the leading supplement experts in the country, his articles online are taken from his best selling book " Supplements For Genetic Growth " which explains how you can increase the number of muscle cells in your body using supplement stacks available at any health food store. This book will demonstrate how the body will try and resist your efforts to grow insane muscles and how, as you age, the ability to create new muscle cells decline so make those cells now! The book is backed by science! This is the most amazing break through in supplement history! The book is available from the LG Sciences website www.lgsciences.com
Creatine: the 2nd Pathway To Genetic Change
Creatine is actually an amino acid like structure that acts as an energy source in skeletal muscles during intense exercise. At rest, creatine is phosphorylated to form phosphocreatine. During intense exercise, this phosphocreatine is utilized by the muscle to form more ATP; which means more energy.
Creatine is not just a source of energy though. Creatine is known to draw water into the muscle cells. This increase in water raises the pressure inside the muscle resulting in a stretching of muscle fibers. Stretch is one of the forces known to cause an increase in protein synthesis in muscle cells. Increased protein synthesis means a positive nitrogen balance and an increase in skeletal muscle growth.
Everyone thinks they know how creatine builds muscle, but they don’t know about a few little-known studies involving creatine and satellite cells. Creatine is known to boost satellite cell production and when taken at the right time (which we will show you later) and in the proper ratio, you can make the most out of your creatine supplementation.
Creatine is reported to increase muscle energy output by recharging ATP and increasing cellular energy output. This is an important aspect of what creatine can do for the bodybuilder or powerlifter, but it isn’t the only story.
Creatine has been shown to be a powerful promoter of satellite cell expression, which again causes new muscle cells to be formed! This is an amazing find, but we researched and realized that creatine timing and ratios are an important part how creatine can be used to build muscle.
Creatine can also be used synergistically with the other nutrients mentioned in this book to build muscle, increase endurance and most importantly help in satellite cell differentiation. We can show you exactly how best to use creatine in the following excerpts from training and nutrient journals.
When picking a good creatine product, look for a mixed creatine supplement using advanced delivery methods.
For example, LG Sciences COLD FUSION EX™ uses a combination of creatine delivery methods to give you the best creatine combination product on the market. It is a mixture of Creatine Ester, Creatine Malate and other Creatine forms to give you a steady, stable release of creatine without the bloat associates with Creatine other creatine products. Cold Fusion EX comes with ingredients to increase stamina and performance, so you get MAXIMUM gains in the gym and the ability to push yourself further than you ever thought possible!
The science behind creatine and satellite cell proliferation is shown in the literature and is summarized below in several abstracts. This is why creatine is the safest and most effective supplement ever created for bodybuilders and currently perfect for anyone under 21. Creatine gives you energy, enhances performance and also has value as an anti-aging supplement for the elderly. It’s sad that creatine has gotten such a bad rap in the press, since it is one of the most safe and effective supplements on the market. The point? Don’t skip on the Creatine when you are looking to build SERIOUS muscle.
Dietary creatine monohydrate supplementation increases satellite cell mitotic activity during compensatory hypertrophy.
Dangott B, Schultz E, Mozdziak PE.Department of Anatomy, University of Wisconsin-Medical School, Madison, USA.
Nutritional status influences muscle growth and athletic performance, but little is known about the effect of nutritional supplements, such as creatine, on satellite cell mitotic activity. The purpose of this study was to examine the effect of oral creatine supplementation on muscle growth, compensatory hypertrophy, and satellite cell mitotic activity. Compensatory hypertrophy was induced in the rat plantaris muscle by removing the soleus and gastrocnemius muscles. Immediately following surgery, a group of six rats was provided with elevated levels of creatine monohydrate in their diet. Another group of six rats was maintained as a non-supplemented control group. Twelve days following surgery, all rats were implanted with mini-osmotic pumps containing the thymidine analog 5-bromo-2'-deoxyuridine (BrdU) to label mitotically active satellite cells. Four weeks after the initial surgery the rats were killed, plantaris muscles were removed and weighed. Subsequently, BrdU-labeled and non-BrdU-labeled nuclei were identified on enzymatically isolated myofiber segments. Muscle mass and myofiber diameters were larger (P < 0.05) in the
muscles that underwent compensatory hypertrophy compared to the control muscles, but there were no differences between muscles from creatine-supplemented and non-creatine-supplemented rats. Similarly, compensatory hypertrophy resulted in an increased (P < 0.05) number of BrdU-labeled myofiber nuclei, but creatine supplementation in combination with compensatory hypertrophy resulted in a higher (P < 0.05) number of BrdU-labeled myofiber nuclei compared to compensatory hypertrophy without creatine supplementation. Thus, creatine supplementation in combination with an increased functional load results in increased satellite cell mitotic activity.
CREATINE SUPPLEMENTATION AUGMENTS THE INCREASE IN SATELLITE CELL AND MYONUCLEI NUMBER IN HUMAN SKELETAL MUSCLE INDUCED BY STRENGTH TRAINING.
Aagaard P.Inst of Sports Medicine Copenhagen.
The present study investigated the influence of creatine and protein supplementation on satellite cell frequency and myonuclei number in human skeletal muscle during 16 wks of heavy-resistance training. In a double-blinded design thirty-two healthy, male subjects (19-26 years) were assigned to strength training (STR) while receiving a timed intake of creatine (STR-CRE) (n=9), protein (STR-PRO) (n=8), placebo (STR-CON) (n=8) or serving as a non-training control group (CON) (n=7). Supplementation was given daily (STR-CRE : 6-24 g creatine monohydrate, STR-PRO: 20 g protein, STR-CON: placebo). Furthermore, timed protein/placebo intake were administered at all training sessions. Muscle biopsies were obtained at wks 0, 4, 8 (wk 8 not CON) and 16 of resistance training (3 days/wk). Satellite cells were identified by immunohistochemistry. Muscle mean fibre (MFA) area was determined after histochemical analysis. All training regimes were found to increase the proportion of satellite cells, however, significantly greater enhancements were observed with creatine supplementation at wk 4 (compared to STR-CON) and at wk 8 (compared to STR-PRO and STR-CON) (p<0.01-0.05). At wk 16, satellite cell number was no longer elevated in STR-CRE, while it remained elevated in STR-PRO and STR-CON. Furthermore, creatine supplementation resulted in an increased number of myonuclei per fibre and increases of 14-17% in MFA at wks 4, 8 and 16 (p<0.01). In contrast, STR-PRO showed increase in MFA only in the later (16 wks, +8%) and STR-CON only in the early (wk 4, +14%) phases of training, respectively (p<0.05). In STR-CRE a positive relationship was found between the percentage increases in MFA and myonuclei from baseline to week 16, respectively (r=0.67, p<0.05). No changes were observed in the control group (CON). In conclusion, the present study demonstrate for the first time that creatine supplementation in combination with strength training amplifies the training-induced increase in satellite cell number and myonuclei concentration in human skeletal muscle fibres, thereby allowing an enhanced muscle fibre growth in response to strength training.
The effects of ergogenic compounds on myogenic satellite cells.
Vierck JL, Icenoggle DL, Bucci L, Dodson MV.Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
PURPOSE: A series of studies were conducted in which compounds commonly shown to be ergogenic aids for strength athletes if taken orally were evaluated for their ability to directly induce postnatal muscle stem cell proliferation or differentiation/fusion in vitro. METHODS: Compounds tested were creatine monohydrate, creatine pyruvate, L-glutamine, dehydroepiandrosterone (DHEA), androstenedione, Ma Huang (Ephedra sinensis) extract, and Zhi Shi (Citrus aurantium) extract. Dulbecco's modified eagle medium, supplemented with minimal levels of serum and antibiotics, was used as the initial vehicle for the test compounds. Subsequently, a defined treatment medium termed ITTC was used. Satellite cells were exposed to the test compounds for the indicated times and then evaluated by counting mononucleated and multinucleated (fused) cells. RESULTS: In serum-containing media, none of the treatment groups displayed increased proliferation over that of the control. However, in the differentiation cultures, 0.10% creatine monohydrate increased differentiation over that of the control cultures. When 0.10% creatine monohydrate was added to defined media formulations, all treatments but one demonstrated increased differentiation over the 0.5% serum control. Time course experiments, which followed the effect of 0.10% creatine monohydrate contained in ITTC defined media over 120 h, suggested that cells exposed to this treatment differentiated earlier and to a greater level than cells exposed to ITTC alone. CONCLUSIONS: Creatine in the monohydrate form induced differentiation of myogenic satellite cells. Other agents examined did not increase satellite cell proliferation or differentiation. These results provide initial evidence for a mechanistic understanding of observed effects in vivo of increased muscular size and strength from creatine supplementation.
Eric D. Marchewitz, is one of the leading supplement experts in the country, his articles online are taken from his best selling book " Supplements For Genetic Growth " which explains how you can increase the number of muscle cells in your body using supplement stacks available at any health food store. This book will demonstrate how the body will try and resist your efforts to grow insane muscles and how, as you age, the ability to create new muscle cells decline so make those cells now! The book is backed by science! This is the most amazing break through in supplement history! The book is available from the LG Sciences website www.lgsciences.com
Creatine is actually an amino acid like structure that acts as an energy source in skeletal muscles during intense exercise. At rest, creatine is phosphorylated to form phosphocreatine. During intense exercise, this phosphocreatine is utilized by the muscle to form more ATP; which means more energy.
Creatine is not just a source of energy though. Creatine is known to draw water into the muscle cells. This increase in water raises the pressure inside the muscle resulting in a stretching of muscle fibers. Stretch is one of the forces known to cause an increase in protein synthesis in muscle cells. Increased protein synthesis means a positive nitrogen balance and an increase in skeletal muscle growth.
Everyone thinks they know how creatine builds muscle, but they don’t know about a few little-known studies involving creatine and satellite cells. Creatine is known to boost satellite cell production and when taken at the right time (which we will show you later) and in the proper ratio, you can make the most out of your creatine supplementation.
Creatine is reported to increase muscle energy output by recharging ATP and increasing cellular energy output. This is an important aspect of what creatine can do for the bodybuilder or powerlifter, but it isn’t the only story.
Creatine has been shown to be a powerful promoter of satellite cell expression, which again causes new muscle cells to be formed! This is an amazing find, but we researched and realized that creatine timing and ratios are an important part how creatine can be used to build muscle.
Creatine can also be used synergistically with the other nutrients mentioned in this book to build muscle, increase endurance and most importantly help in satellite cell differentiation. We can show you exactly how best to use creatine in the following excerpts from training and nutrient journals.
When picking a good creatine product, look for a mixed creatine supplement using advanced delivery methods.
For example, LG Sciences COLD FUSION EX™ uses a combination of creatine delivery methods to give you the best creatine combination product on the market. It is a mixture of Creatine Ester, Creatine Malate and other Creatine forms to give you a steady, stable release of creatine without the bloat associates with Creatine other creatine products. Cold Fusion EX comes with ingredients to increase stamina and performance, so you get MAXIMUM gains in the gym and the ability to push yourself further than you ever thought possible!
The science behind creatine and satellite cell proliferation is shown in the literature and is summarized below in several abstracts. This is why creatine is the safest and most effective supplement ever created for bodybuilders and currently perfect for anyone under 21. Creatine gives you energy, enhances performance and also has value as an anti-aging supplement for the elderly. It’s sad that creatine has gotten such a bad rap in the press, since it is one of the most safe and effective supplements on the market. The point? Don’t skip on the Creatine when you are looking to build SERIOUS muscle.
Dietary creatine monohydrate supplementation increases satellite cell mitotic activity during compensatory hypertrophy.
Dangott B, Schultz E, Mozdziak PE.Department of Anatomy, University of Wisconsin-Medical School, Madison, USA.
Nutritional status influences muscle growth and athletic performance, but little is known about the effect of nutritional supplements, such as creatine, on satellite cell mitotic activity. The purpose of this study was to examine the effect of oral creatine supplementation on muscle growth, compensatory hypertrophy, and satellite cell mitotic activity. Compensatory hypertrophy was induced in the rat plantaris muscle by removing the soleus and gastrocnemius muscles. Immediately following surgery, a group of six rats was provided with elevated levels of creatine monohydrate in their diet. Another group of six rats was maintained as a non-supplemented control group. Twelve days following surgery, all rats were implanted with mini-osmotic pumps containing the thymidine analog 5-bromo-2'-deoxyuridine (BrdU) to label mitotically active satellite cells. Four weeks after the initial surgery the rats were killed, plantaris muscles were removed and weighed. Subsequently, BrdU-labeled and non-BrdU-labeled nuclei were identified on enzymatically isolated myofiber segments. Muscle mass and myofiber diameters were larger (P < 0.05) in the
muscles that underwent compensatory hypertrophy compared to the control muscles, but there were no differences between muscles from creatine-supplemented and non-creatine-supplemented rats. Similarly, compensatory hypertrophy resulted in an increased (P < 0.05) number of BrdU-labeled myofiber nuclei, but creatine supplementation in combination with compensatory hypertrophy resulted in a higher (P < 0.05) number of BrdU-labeled myofiber nuclei compared to compensatory hypertrophy without creatine supplementation. Thus, creatine supplementation in combination with an increased functional load results in increased satellite cell mitotic activity.
CREATINE SUPPLEMENTATION AUGMENTS THE INCREASE IN SATELLITE CELL AND MYONUCLEI NUMBER IN HUMAN SKELETAL MUSCLE INDUCED BY STRENGTH TRAINING.
Aagaard P.Inst of Sports Medicine Copenhagen.
The present study investigated the influence of creatine and protein supplementation on satellite cell frequency and myonuclei number in human skeletal muscle during 16 wks of heavy-resistance training. In a double-blinded design thirty-two healthy, male subjects (19-26 years) were assigned to strength training (STR) while receiving a timed intake of creatine (STR-CRE) (n=9), protein (STR-PRO) (n=8), placebo (STR-CON) (n=8) or serving as a non-training control group (CON) (n=7). Supplementation was given daily (STR-CRE : 6-24 g creatine monohydrate, STR-PRO: 20 g protein, STR-CON: placebo). Furthermore, timed protein/placebo intake were administered at all training sessions. Muscle biopsies were obtained at wks 0, 4, 8 (wk 8 not CON) and 16 of resistance training (3 days/wk). Satellite cells were identified by immunohistochemistry. Muscle mean fibre (MFA) area was determined after histochemical analysis. All training regimes were found to increase the proportion of satellite cells, however, significantly greater enhancements were observed with creatine supplementation at wk 4 (compared to STR-CON) and at wk 8 (compared to STR-PRO and STR-CON) (p<0.01-0.05). At wk 16, satellite cell number was no longer elevated in STR-CRE, while it remained elevated in STR-PRO and STR-CON. Furthermore, creatine supplementation resulted in an increased number of myonuclei per fibre and increases of 14-17% in MFA at wks 4, 8 and 16 (p<0.01). In contrast, STR-PRO showed increase in MFA only in the later (16 wks, +8%) and STR-CON only in the early (wk 4, +14%) phases of training, respectively (p<0.05). In STR-CRE a positive relationship was found between the percentage increases in MFA and myonuclei from baseline to week 16, respectively (r=0.67, p<0.05). No changes were observed in the control group (CON). In conclusion, the present study demonstrate for the first time that creatine supplementation in combination with strength training amplifies the training-induced increase in satellite cell number and myonuclei concentration in human skeletal muscle fibres, thereby allowing an enhanced muscle fibre growth in response to strength training.
The effects of ergogenic compounds on myogenic satellite cells.
Vierck JL, Icenoggle DL, Bucci L, Dodson MV.Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
PURPOSE: A series of studies were conducted in which compounds commonly shown to be ergogenic aids for strength athletes if taken orally were evaluated for their ability to directly induce postnatal muscle stem cell proliferation or differentiation/fusion in vitro. METHODS: Compounds tested were creatine monohydrate, creatine pyruvate, L-glutamine, dehydroepiandrosterone (DHEA), androstenedione, Ma Huang (Ephedra sinensis) extract, and Zhi Shi (Citrus aurantium) extract. Dulbecco's modified eagle medium, supplemented with minimal levels of serum and antibiotics, was used as the initial vehicle for the test compounds. Subsequently, a defined treatment medium termed ITTC was used. Satellite cells were exposed to the test compounds for the indicated times and then evaluated by counting mononucleated and multinucleated (fused) cells. RESULTS: In serum-containing media, none of the treatment groups displayed increased proliferation over that of the control. However, in the differentiation cultures, 0.10% creatine monohydrate increased differentiation over that of the control cultures. When 0.10% creatine monohydrate was added to defined media formulations, all treatments but one demonstrated increased differentiation over the 0.5% serum control. Time course experiments, which followed the effect of 0.10% creatine monohydrate contained in ITTC defined media over 120 h, suggested that cells exposed to this treatment differentiated earlier and to a greater level than cells exposed to ITTC alone. CONCLUSIONS: Creatine in the monohydrate form induced differentiation of myogenic satellite cells. Other agents examined did not increase satellite cell proliferation or differentiation. These results provide initial evidence for a mechanistic understanding of observed effects in vivo of increased muscular size and strength from creatine supplementation.
Eric D. Marchewitz, is one of the leading supplement experts in the country, his articles online are taken from his best selling book " Supplements For Genetic Growth " which explains how you can increase the number of muscle cells in your body using supplement stacks available at any health food store. This book will demonstrate how the body will try and resist your efforts to grow insane muscles and how, as you age, the ability to create new muscle cells decline so make those cells now! The book is backed by science! This is the most amazing break through in supplement history! The book is available from the LG Sciences website www.lgsciences.com
LG Sciences Creatine Part 1
The market is starting to get flooded with many different kinds of creatine. Our company LG Sciences has a creatine complex, Cold Fusion EX. Most products are pretty good, but what separates many of them is effectiveness and taste. Cold Fusion EX is formulated to be the best tasting creatine complex on the market and it has ingredients that make it up to six times more effective than regular creatine. That being said almost any creatine product will give results, even straight monohydrate! Depending on the type, most creatine is taken at 3-10g per day and that seems like an effective dose.With all of the choices out there how can we decide what is the best form if such a thing actually exists. Creatine is an amino acid that is naturally occurring in the body and provides cellular energy and a host of other benefits to both the bodybuilder and general health enthusiast. In fact creatine is even being looked at to provide increased energy and wellness benefits for the elderly. It may provide heart benefits and also increase mental acuity for people that have diseases like Alzheimer’s. Creatine is responsible for turning ADP into ATP which is your body’s main energy supply in the mitochondria. ATP is split to form ADP (losing a phosphate to create energy) and Creatine Phosphate “hangs around” to recharge the ADP molecule so it can be used to create energy again. Any Creatine is converted to Creatine Phosphate in the body, but oddly enough taking Creatine Phosphate as a supplement never really gave the kind of results that were achieved by many other creatine types, so it was effectively scrapped. Creatine is the base product for any bodybuilder looking to increase size and strength. It adds well to any other product and the only thing more basic than creatine is a protein supplement. Creatine helps the muscle cell hold more water, which can expand the fascia of the muscle increasing its volume. So, creatine is useful for strength and stamina by recharging the muscle energy system, it also increases new muscle cells (monohydrate for sure) and increases pumps for a muscle stretching effect. It is the most popular and beneficial supplement ever for bodybuilders.Here is a basic list of just some of the Creatines on the market:Creatine Monohydrate – this is the original version sold back in the 80’s for improving strength and stamina for weightlifters. It is tried and tested, however it has been replaced with newer and fancier creatine molecules. I HIGHLY suggest supplements still contain some creatine monohydrate for one big reason. Creatine monohydrate is the only form proven in scientific studies to create new muscle cells. There are many studies showing Creatine Monohydrate increases the formation of new muscle cells and one important study showing that other forms of creatine do not have this effect!Creatine Malate – this is creatine bound to malic acid. The preferred form is DiCreatine Malate since that is the only form that is possible, the other forms (tricreatine malate) are usually just creatine monohydrate mixed with straight malic acid. The supposed benefit of Creatine Malate is reduced bloating and increased endurance, since malate is involved in increasing cellular energy by being part of the krebs cycle. Overall, this ingredient has many years of solid anecdotal support and I feel it is a good addition to any creatine blend.Creatine Asperate – this is creatine bound to aspartic acid. This is very similar to Creatine Malate and should prove to be even better for endurance athletes, since asparate is even more directly involved in recharging the mitochondrial energy system. Creatine Asperate is an improved form of Creatine Malate, but both should give very good endurance gains without extra bloat.Creatine Ethyl Ester HCL – the latest “big splash” is Creatine Ethyl Ester. This ingredient has some strong anecdotal evidence of it working, but the science behind it is very flawed. The “ester” is supposed to make the creatine more absorbable and fat soluble. This belief is pretty flawed since creatine dissolves quite easily in water and there is little chance of it becoming fat soluable with this ester even if it was desirable to do so. Most likely this product works by stabilizing the Creatine molecule with the HCL portion, which slows down some of the conversion to creatinine (a waste product).Creatine Gluconate – this product is simply Creatine bonded to sugar or glucose. No idea why that would be a big advantage over dumping some sugar in with your monohydrate, but it certainly doesn’t hurt anything. Sugar helps the uptake of creatine in the cell, so it could have a positive effect.Creatine Decanoate – no clue why anyone would want to bond decanoic acid to creatine unless they just want to take advantage of the “deca” in the name. Decanoic acid doesn’t seem to increase performance or provide any benefits.Creatine AKG – simply creatine bound to alpha keto gluterate which is a glutamine type molecule. No real data on this ingredient, but it should work pretty well in theory since both creatine and glutamine are good for you. AKG should help with the absorption of creatine.Creatine Magnesium Chelate – another creatine molecule bound to magnesium. Chelated minerals were very popular as a way of increasing the absorption. Certainly nothing wrong with magnesium, since it is great for you, but I don’t see the positive benefit of binding creatine to a mineral when you could just take a good magnesium supplement and plain old monohydrate.Creatine Orotate – similar to Creatine Malate and Creatine Asperate, Creatine Orotate provides increased cellular energy and improved absorption. Orotic Acid does provide benefit to the bodybuilder by increasing strength and stamina.Well, that is a short list of the types of creatines that are on the market. In my opinion a mixed form of multiple creatines is the best way to take a creatine supplement. I would for sure have some Creatine Monohydrate in with your other mixes, since as stated above only Creatine Monohydrate is proven in the scientific literature to actually increase satellite cells (new muscle growth) where some of the other products like Creatine Malate did not have this effect. Without new muscle cells, a bodybuilder can only get so big, which is why some people are “hard gainers”.In the next installment I will discuss various delivery methods that are used to improve creatine uptake. Until then, happy lifting!Eric Marchewitz is affiliated with LG Sciences which has a mixed creatine product called Cold Fusion EX. It is a very advanced creatine complex that provides increased strength and stamina and a delicious taste. You can read more about Cold Fusion EX on www.lgsciences.com
The market is starting to get flooded with many different kinds of creatine. Our company LG Sciences has a creatine complex, Cold Fusion EX. Most products are pretty good, but what separates many of them is effectiveness and taste. Cold Fusion EX is formulated to be the best tasting creatine complex on the market and it has ingredients that make it up to six times more effective than regular creatine. That being said almost any creatine product will give results, even straight monohydrate! Depending on the type, most creatine is taken at 3-10g per day and that seems like an effective dose.With all of the choices out there how can we decide what is the best form if such a thing actually exists. Creatine is an amino acid that is naturally occurring in the body and provides cellular energy and a host of other benefits to both the bodybuilder and general health enthusiast. In fact creatine is even being looked at to provide increased energy and wellness benefits for the elderly. It may provide heart benefits and also increase mental acuity for people that have diseases like Alzheimer’s. Creatine is responsible for turning ADP into ATP which is your body’s main energy supply in the mitochondria. ATP is split to form ADP (losing a phosphate to create energy) and Creatine Phosphate “hangs around” to recharge the ADP molecule so it can be used to create energy again. Any Creatine is converted to Creatine Phosphate in the body, but oddly enough taking Creatine Phosphate as a supplement never really gave the kind of results that were achieved by many other creatine types, so it was effectively scrapped. Creatine is the base product for any bodybuilder looking to increase size and strength. It adds well to any other product and the only thing more basic than creatine is a protein supplement. Creatine helps the muscle cell hold more water, which can expand the fascia of the muscle increasing its volume. So, creatine is useful for strength and stamina by recharging the muscle energy system, it also increases new muscle cells (monohydrate for sure) and increases pumps for a muscle stretching effect. It is the most popular and beneficial supplement ever for bodybuilders.Here is a basic list of just some of the Creatines on the market:Creatine Monohydrate – this is the original version sold back in the 80’s for improving strength and stamina for weightlifters. It is tried and tested, however it has been replaced with newer and fancier creatine molecules. I HIGHLY suggest supplements still contain some creatine monohydrate for one big reason. Creatine monohydrate is the only form proven in scientific studies to create new muscle cells. There are many studies showing Creatine Monohydrate increases the formation of new muscle cells and one important study showing that other forms of creatine do not have this effect!Creatine Malate – this is creatine bound to malic acid. The preferred form is DiCreatine Malate since that is the only form that is possible, the other forms (tricreatine malate) are usually just creatine monohydrate mixed with straight malic acid. The supposed benefit of Creatine Malate is reduced bloating and increased endurance, since malate is involved in increasing cellular energy by being part of the krebs cycle. Overall, this ingredient has many years of solid anecdotal support and I feel it is a good addition to any creatine blend.Creatine Asperate – this is creatine bound to aspartic acid. This is very similar to Creatine Malate and should prove to be even better for endurance athletes, since asparate is even more directly involved in recharging the mitochondrial energy system. Creatine Asperate is an improved form of Creatine Malate, but both should give very good endurance gains without extra bloat.Creatine Ethyl Ester HCL – the latest “big splash” is Creatine Ethyl Ester. This ingredient has some strong anecdotal evidence of it working, but the science behind it is very flawed. The “ester” is supposed to make the creatine more absorbable and fat soluble. This belief is pretty flawed since creatine dissolves quite easily in water and there is little chance of it becoming fat soluable with this ester even if it was desirable to do so. Most likely this product works by stabilizing the Creatine molecule with the HCL portion, which slows down some of the conversion to creatinine (a waste product).Creatine Gluconate – this product is simply Creatine bonded to sugar or glucose. No idea why that would be a big advantage over dumping some sugar in with your monohydrate, but it certainly doesn’t hurt anything. Sugar helps the uptake of creatine in the cell, so it could have a positive effect.Creatine Decanoate – no clue why anyone would want to bond decanoic acid to creatine unless they just want to take advantage of the “deca” in the name. Decanoic acid doesn’t seem to increase performance or provide any benefits.Creatine AKG – simply creatine bound to alpha keto gluterate which is a glutamine type molecule. No real data on this ingredient, but it should work pretty well in theory since both creatine and glutamine are good for you. AKG should help with the absorption of creatine.Creatine Magnesium Chelate – another creatine molecule bound to magnesium. Chelated minerals were very popular as a way of increasing the absorption. Certainly nothing wrong with magnesium, since it is great for you, but I don’t see the positive benefit of binding creatine to a mineral when you could just take a good magnesium supplement and plain old monohydrate.Creatine Orotate – similar to Creatine Malate and Creatine Asperate, Creatine Orotate provides increased cellular energy and improved absorption. Orotic Acid does provide benefit to the bodybuilder by increasing strength and stamina.Well, that is a short list of the types of creatines that are on the market. In my opinion a mixed form of multiple creatines is the best way to take a creatine supplement. I would for sure have some Creatine Monohydrate in with your other mixes, since as stated above only Creatine Monohydrate is proven in the scientific literature to actually increase satellite cells (new muscle growth) where some of the other products like Creatine Malate did not have this effect. Without new muscle cells, a bodybuilder can only get so big, which is why some people are “hard gainers”.In the next installment I will discuss various delivery methods that are used to improve creatine uptake. Until then, happy lifting!Eric Marchewitz is affiliated with LG Sciences which has a mixed creatine product called Cold Fusion EX. It is a very advanced creatine complex that provides increased strength and stamina and a delicious taste. You can read more about Cold Fusion EX on www.lgsciences.com
Growth Hormone and I-GF-1
I am not a huge fan of synthetic growth hormone, but I recognize that what separates the TRULY HUGE from the average guy is the stacking of Growth Hormone (GH) with androgens. Growth hormone on its own doesn’t seem to really add a ton of size on you though and it does carry some risks, so it’s not the best choice in my opinion for a stand alone product. It will however really increase satellite cells over straight androgen boosting alone and other supplements. The major problem with GH on a cycle is that it won’t put tons of size on your right away. We make a GH product (IGH-1) that you can take to increase your own GH production but it will not pack size on you instantly. If you are not committed to the long haul, then honestly, GH products are not for you. If you want to put on muscle that lasts and is with you for life, then a good GH product will help you get to that goal. Even REAL GH cycles are gaged in terms of months of use, not days. GH cycles last for 6 months and you don’t see real results for at least 45 days. This is why that a GH releaser is not going to pack on pounds of muscle in 15 days like a cycle of prohormones, even 4 iu of GH injections will take a long time to see the effect you desire. That isn’t to say that GH isn’t EXTREMELY useful, it is more like an important part of a good long term plan vs. androgens that give an immediate effect that feels damn good. I take a GH releaser every night, since reading the studies PROVING that GH is a valuable part of changing your genetics.When 1+1=3When you combine growth hormone with a powerful androgen, you get some serious muscle building, because GH increases satellite cell proliferation, gets you lean and increases IGF-1 production. IGF-1 builds muscle and increases satellite cell expression while burning fat. This is what makes growth hormone worth the 300-400 dollars per week the professionals spend on a cycle, even before IGF-1 was available. Growth hormone burns fat, but also greatly increases IGF-1 levels, which if you are in the circle of knowledge is VERY important, since IGF-1 is a natural anabolic.Most of the IGF-1 on the market today is either fake or worthless, because IGF-1 must be cold packed and shipped immediately. IGF-1 degrades very quickly, but if you are lucky enough to get your hands on some REAL IGF-1, you are in for a treat. Expect to pay at least $300 per week though for the real stuff.IGF-1 and GH can be stimulated by the body, meaning that your natural levels can be increased, which gives you the ability to get most of the same results as a pro bodybuilder without the expense or risk. GH secretagogues (things that increase your natural production or secretion) are on the market and they should at least include things like ArgininePryoglutamate, Ornithine, Astragulus, and L-Dopa. These are the most potent Growth Hormone secretagogues on the market and can sky rocket your GH levels, allowing you to build slabs of muscle. GH is pretty easy to increase with the use of secretagogues, but IGF-1 is very tough to boost naturally. There is only one ingredient I know that increases IGF-1 naturally. This is in products like LG Sciences Formadrol Extreme pictured above, and the ingredient is Daidzein, which is the only known naturally occurring IGF-1 secretagogue. So how does GH increase satellite expression? Again, let’s look at the literature.GH and IGF-1 on Satellite Cell ExpressionEffects of growth hormone on skeletal muscle. I. Studies on normal adult rats.Ullman M, Oldfors A.Department of Pathology, Gothenburg University, Sweden.A study was made on the effects of recombinant human growth hormone (rhGH) on fast and slow skeletal muscle in normal adult female rats. Daily injections of 4 IE of rhGH over 36 days resulted in increased levels of insulin-like growth factor I in serum and increased body weight. Morphometric analysis of the muscle fibres of the extensor digitorum longus (EDL) and soleus muscles revealed a significant increase in diameter of both type 1 and type 2 fibres in both muscles. The DNA: protein ratio and the number of satellite cells:muscle fibre in cross-sections was increased in the GH-treated rats in relation to controls. The results show that rhGH has pronounced effects on both cell proliferation and muscle fibre growth in skeletal muscle of normal adults rats.IGF-1 induces human myotube hypertrophy by increasing cell recruitment.Jacquemin V, Furling D, Bigot A, Butler-Browne GS, Mouly V.CNRS UMR 7000 Cytosquelette et Developpement, Paris, France.Insulin-like growth factor-1 (IGF-1) has been shown in rodents (i) in vivo to induce muscle fiber hypertrophy and to prevent muscle mass decline with age and (ii) in vitro to enhance the proliferative life span of myoblasts and to induce myotube hypertrophy. In this study, performed on human primary cultures, we have shown that IGF-1 has very little effect on the proliferative life span of human myoblasts but does delay replicative senescence. IGF-1 also induces hypertrophy of human myotubes in vitro, as characterized by an increase in the mean number of nuclei per myotube, an increase in the fusion index, and an increase in myosin heavy chain (MyHC) content. In addition, muscle hypertrophy can be triggered in the absence of proliferation by recruiting more mononucleated cells. We propose that IGF-1-induced hypertrophy can involve the recruitment of reserve cells in human skeletal muscle.Insulin-like growth factor 1 and muscle growth: implication for satellite cell proliferation.Machida S, Booth FW.Department of Biomedical Sciences, University of Missouri-Columbia, E102 Veterinary Medical Building, 1600 East Rollins Road, Columbia, MO 65211, USA.Insulin-like growth factor 1 (IGF-1) has been shown to rescue the aging-related or inactivity-induced loss of muscle mass through the activation of satellite cells. However, the signalling pathways and the mechanism by which IGF-1 affects satellite cells have not been not completely identified. The purpose of the present review is to provide current understanding of the cellular and molecular events underlying IGF-1 induced proliferation of satellite cells.Expression and splicing of the insulin-like growth factor gene in rodent muscle is associated with muscle satellite (stem) cell activation following local tissue damage.Hill M, Goldspink G.Basic Medical Sciences and Department of Surgery, Royal Free and University College Medical School, Royal Free Campus, Rowland Hill Street, London NW3 2PF, UK.Muscle satellite cells are mononuclear cells that remain in a quiescent state until activated when they proliferate and fuse with muscle fibres to donate nuclei, a process necessary for post-embryonic growth, hypertrophy and tissue repair in this post-mitotic tissue. These processes have been associated with expression of the insulin-like growth factor (IGF-I) gene that can undergo alternative splicing to generate different gene products with varying functions. To gain insight into the cellular mechanisms involved in local tissue repair, the time courses of expression of two IGF-I splice variants produced in muscle were determined together with marker genes for satellite cell activation following local muscle damage. Using real-time RT-PCR with specific primers, the mRNA transcripts in rat tibialis anterior muscles were measured at different time intervals following either mechanical damage imposed by electrical stimulation of the stretched muscle or damage caused by injection with bupivacaine. It was found that the autocrine splice variant mechano growth factor (MGF) was rapidly expressed and then declined within a few days following both types of damage. Systemic IGF-IEa was more slowly upregulated and its increase was commensurate with the rate of decline in MGF expression. Satellite cell activation as measured by M-cadherin and one of the muscle regulatory factors MyoD and the sequence of expression suggests that the initial pulse of MGF is responsible for satellite cell activation, as the systemic IGF-IEa mRNA expression peaks after the expression of these markers, including M-cadherin protein. Later splicing of the IGF-I gene away from MGF but towards IGF-IEa seems physiologically appropriate as IGF-IEa is the main source of mature IGF-I for upregulation of protein synthesis required to complete the repair.GH on Muscle:Growth hormone / insulin-like growth factor-1 axis during puberty.Christoforidis A, Maniadaki I, Stanhope R.Department of Endocrinology,Great Ormond Street Hospital and the Middlesex Hospital (UCLH), London, UK.Puberty is a dynamic, transitional period of life which is characterized by the acquisition of secondary sexual characteristics leading to the development of fertility. Puberty is accompanied by sexually dimorphic changes in linear growth, body proportions and body composition. The pubertal growth spurt is influenced by a number of factors such as hormones, nutrition, physical activity and general health, acting mostly in concert in order to modify a genetic potential for growth. Growth hormone (GH) is traditionally considered to be the main regulator of growth. During puberty, elevated sex steroid concentrations (especially oestrogens) stimulate GH production, leading to an activation of the whole GH/Insulinlike growth factor-1 (IGF-1) axis. This activation is mostly characterized by an increase in the amplitude of GH pulses rather than an increase in frequency or in duration. Interactions between GH and sex steroids (especially androgens) express an anabolic effect on muscle mass, bone mineralization and body proportion which constitutes the male and the female adult body composition.Intact insulin and insulin-like growth factor-I receptor signaling is required for growth hormone effects on skeletal muscle growth and function in vivo.Kim H, Barton E, Muja N, Yakar S, Pennisi P, Leroith D.Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Building 10, Room 8D12, Bethesda, Maryland 20892-1758, USA.GH and IGF-I are potent regulators of muscle growth and function. Although IGF-I is known to mediate many of the effects of GH, it is not yet clear whether all effects of GH are completely dependent on the IGF-I system. To evaluate the biological effects of the GH/IGF-I axis on muscle growth, we administrated recombinant human GH to mice, which lack IGF-I function specifically in skeletal muscle, due to the overexpression of a dominant-negative IGF-I receptor in this tissue (MKR mice). GH treatment significantly increased the levels of hepatic IGF-I mRNA and serum IGF-I levels in both wild-type (WT) and MKR mice. These GH-induced effects were paralleled by increases in body weight and in the weights of most GH-responsive organs in both groups of mice. Interestingly, unlike WT mice, GH treatment had no effect on skeletal muscle weight in MKR mice. GH treatment failed to reverse the impaired muscle function in MKR mice. Furthermore, MKR mice exhibited no effects of GH on the cross-sectional area of myofibers and the proliferation of satellite cells. Taken together, these data suggest that the in vivo effects of GH on muscle mass and strength are primarily mediated by activation of the IGF-I receptor.Insulin-like growth factor 1 and muscle growth: implication for satellite cell proliferation.Machida S, Booth FW.Department of Biomedical Sciences, University of Missouri-Columbia, E102 Veterinary Medical Building, 1600 East Rollins Road, Columbia, MO 65211, USA.Insulin-like growth factor 1 (IGF-1) has been shown to rescue the aging-related or inactivity-induced loss of muscle mass through the activation of satellite cells. However, the signalling pathways and the mechanism by which IGF-1 affects satellite cells have not been not completely identified. The purpose of the present review is to provide current understanding of the cellular and molecular events underlying IGF-1 induced proliferation of satellite cells.The effects of growth hormone and/or testosterone in healthy elderly men: a randomized controlled trial.Giannoulis MG, Sonksen PH, Umpleby M, Breen L, Pentecost C, Whyte M, McMillan CV, Bradley C, Martin FC.Department of Diabetes and Endocrinology, GKT School of Medicine, King's College London, St. Thomas' Hospital, London SE1 7EH, UK.CONTEXT:Declines in GH and testosterone (Te) secretion may contribute to the detrimental aging changes of elderly men. OBJECTIVE: To assess the effects of near-physiological GH with/without Te administration on lean body mass, total body fat, midthigh muscle cross-section area, muscle strength, aerobic capacity, condition-specific quality of life (Age-Related Hormone Deficiency-Dependent Quality of Life questionnaire), and generic health status (36-Item Short-Form Health Survey) of older men. DESIGN, SETTINGS, AND PARTICIPANTS: A 6-month, randomized, double-blind, placebo-controlled trial was performed on 80 healthy, community-dwelling, older men (age, 65-80 yr). INTERVENTIONS: Participants were randomized to receive 1) placebo GH or placebo Te, 2) recombinant human GH (rhGH) and placebo Te (GH), 3) Te and placebo rhGH (Te), or 4) rhGH and Te (GHTe). GH doses were titrated over 8 wk to produce IGF-I levels in the upper half of the age-specific reference range. A fixed dose of Te (5 mg) was given by transdermal patches. RESULTS: Lean body mass increased with GHTe (P = 0.008) and GH (P = 0.004), compared with placebo. Total body fat decreased with GHTe only (P = 0.02). Midthigh muscle (P = 0.006) and aerobic capacity (P < p =" 0.007)" p =" 0.003)." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstract&term=">Ehrnborg C, Ellegard L, Bosaeus I, Bengtsson BA, Rosen T.Research Centre for Endocrinology and Metabolism, Department of Internal Medicine, Sahlgrenska University Hospital, S-413 45 Goteborg, Sweden. christer.ehrnborg@medic.gu.seOBJECTIVES: To study the effects on body composition after 1 month's administration of supraphysiological doses of growth hormone (GH) in healthy, active young adults with normal GH-IGF-I axis. SUBJECTS AND METHODS: Thirty healthy, physically active volunteers (15 men and 15 women), mean age 25.9 years (range 18-35), participated in this study, designed as a randomized, double-blind, placebo-controlled, parallel study with three groups (n = 10: five men and five women in each group). The groups comprised the following: placebo (P), GH 0.1 IU/kg/day [0.033 mg/kg/day] (GH 0.1) and GH 0.2 IU/kg/day [0.067 mg/kg/day] (GH 0.2). RESULTS: In the pooled group with active GH treatment (n = 20) the results showed significant increases: IGF-I increased by 134% (baseline vs. after 1 month), body weight by 2.7%, fat free mass by 5.3%, total body water by 6.5% and extracellular water (ECW) by 9.6%. Body fat decreased significantly by 6.6%. No significant change in intracellular water was detected. The observed increase in fat free mass by 5.3% was explained by the ECW increase, indicating limited anabolic effects of the supraphysiological GH doses. Changes were noticeable in both genders, although more prominent in the male subjects. Fluid retention symptoms occurred in the majority of individuals. CONCLUSIONS: This is, to our knowledge, the first placebo-controlled trial to show the effects of supraphysiological GH doses on body composition and IGF-I levels in physically active and healthy individuals of both genders; the results indicate limited anabolic effects of GH with these supraphysiological doses. The role of GH as an effective anabolic-doping agent is questioned.GH alone is not enough, which is why we stack…Here is an example of an effective stacking of compatible supplements to achieve a specific objective.Regulating of growth hormone sensitivity by sex steroids: implications for therapy.KK, Gibney J, Johannsson G, Wolthers T.Pituitary Research Unit, Garvan Institute of Medical Research and Department of Endocrinology, St. Vincent's Hospital, Sydney, Australia.Growth hormone (GH) is an important regulator of body composition, reducing body fat by stimulating fat oxidation and enhancing lean body mass by stimulating protein accretion. The emergence of differences in body composition between the sexes during puberty suggests sex steroids modulate the action of GH. Work from our laboratory have investigated the influence of estrogens and androgens on the metabolic actions of GH in human adults. The liver is an important site of physiological interaction as it is a sex steroid responsive organ and a major target of GH action. Estrogen, when administered orally impairs the GHregulated endocrine and metabolic function of the liver via a first-pass effect. It reduces circulating IGF-I, fat oxidation and protein synthesis, contributing to a loss of lean and a gain of fat mass. These effects occur in normal and in GH-deficient women and are avoided by transdermal administration of physiological doses of estrogen. In contrast, studies in hypopituitary men indicate that testosterone enhances the metabolic effects of GH. Testosterone alone stimulates fat oxidation and protein synthesis, both of which are enhanced by GH. Studies in GH deficiency adults have consistently reported women to be less sensitive to GH than men. In summary, estrogens and androgens exert divergent effects on the action of GH. The results provide an explanation for sexual dimorphism in body composition in adults and the genderrelated response to GH replacement in hypopituitary subjects. In the management of hypopituitarism, estrogens should be administered by the parenteral route in women and testosterone be replaced in men to optimize the benefits of GH replacement.Eric D. Marchewitz, is one of the leading supplement experts in the country, his articles online are taken from his best selling book " Supplements For Genetic Growth " which explains how you can increase the number of muscle cells in your body using supplement stacks available at any health food store. This book will demonstrate how the body will try and resist your efforts to grow insane muscles and how, as you age, the ability to create new muscle cells decline so make those cells now! The book is backed by science! This is the most amazing break through in supplement history! The book is available from the LG Sciences website http://www.lgsciences.com/
I am not a huge fan of synthetic growth hormone, but I recognize that what separates the TRULY HUGE from the average guy is the stacking of Growth Hormone (GH) with androgens. Growth hormone on its own doesn’t seem to really add a ton of size on you though and it does carry some risks, so it’s not the best choice in my opinion for a stand alone product. It will however really increase satellite cells over straight androgen boosting alone and other supplements. The major problem with GH on a cycle is that it won’t put tons of size on your right away. We make a GH product (IGH-1) that you can take to increase your own GH production but it will not pack size on you instantly. If you are not committed to the long haul, then honestly, GH products are not for you. If you want to put on muscle that lasts and is with you for life, then a good GH product will help you get to that goal. Even REAL GH cycles are gaged in terms of months of use, not days. GH cycles last for 6 months and you don’t see real results for at least 45 days. This is why that a GH releaser is not going to pack on pounds of muscle in 15 days like a cycle of prohormones, even 4 iu of GH injections will take a long time to see the effect you desire. That isn’t to say that GH isn’t EXTREMELY useful, it is more like an important part of a good long term plan vs. androgens that give an immediate effect that feels damn good. I take a GH releaser every night, since reading the studies PROVING that GH is a valuable part of changing your genetics.When 1+1=3When you combine growth hormone with a powerful androgen, you get some serious muscle building, because GH increases satellite cell proliferation, gets you lean and increases IGF-1 production. IGF-1 builds muscle and increases satellite cell expression while burning fat. This is what makes growth hormone worth the 300-400 dollars per week the professionals spend on a cycle, even before IGF-1 was available. Growth hormone burns fat, but also greatly increases IGF-1 levels, which if you are in the circle of knowledge is VERY important, since IGF-1 is a natural anabolic.Most of the IGF-1 on the market today is either fake or worthless, because IGF-1 must be cold packed and shipped immediately. IGF-1 degrades very quickly, but if you are lucky enough to get your hands on some REAL IGF-1, you are in for a treat. Expect to pay at least $300 per week though for the real stuff.IGF-1 and GH can be stimulated by the body, meaning that your natural levels can be increased, which gives you the ability to get most of the same results as a pro bodybuilder without the expense or risk. GH secretagogues (things that increase your natural production or secretion) are on the market and they should at least include things like ArgininePryoglutamate, Ornithine, Astragulus, and L-Dopa. These are the most potent Growth Hormone secretagogues on the market and can sky rocket your GH levels, allowing you to build slabs of muscle. GH is pretty easy to increase with the use of secretagogues, but IGF-1 is very tough to boost naturally. There is only one ingredient I know that increases IGF-1 naturally. This is in products like LG Sciences Formadrol Extreme pictured above, and the ingredient is Daidzein, which is the only known naturally occurring IGF-1 secretagogue. So how does GH increase satellite expression? Again, let’s look at the literature.GH and IGF-1 on Satellite Cell ExpressionEffects of growth hormone on skeletal muscle. I. Studies on normal adult rats.Ullman M, Oldfors A.Department of Pathology, Gothenburg University, Sweden.A study was made on the effects of recombinant human growth hormone (rhGH) on fast and slow skeletal muscle in normal adult female rats. Daily injections of 4 IE of rhGH over 36 days resulted in increased levels of insulin-like growth factor I in serum and increased body weight. Morphometric analysis of the muscle fibres of the extensor digitorum longus (EDL) and soleus muscles revealed a significant increase in diameter of both type 1 and type 2 fibres in both muscles. The DNA: protein ratio and the number of satellite cells:muscle fibre in cross-sections was increased in the GH-treated rats in relation to controls. The results show that rhGH has pronounced effects on both cell proliferation and muscle fibre growth in skeletal muscle of normal adults rats.IGF-1 induces human myotube hypertrophy by increasing cell recruitment.Jacquemin V, Furling D, Bigot A, Butler-Browne GS, Mouly V.CNRS UMR 7000 Cytosquelette et Developpement, Paris, France.Insulin-like growth factor-1 (IGF-1) has been shown in rodents (i) in vivo to induce muscle fiber hypertrophy and to prevent muscle mass decline with age and (ii) in vitro to enhance the proliferative life span of myoblasts and to induce myotube hypertrophy. In this study, performed on human primary cultures, we have shown that IGF-1 has very little effect on the proliferative life span of human myoblasts but does delay replicative senescence. IGF-1 also induces hypertrophy of human myotubes in vitro, as characterized by an increase in the mean number of nuclei per myotube, an increase in the fusion index, and an increase in myosin heavy chain (MyHC) content. In addition, muscle hypertrophy can be triggered in the absence of proliferation by recruiting more mononucleated cells. We propose that IGF-1-induced hypertrophy can involve the recruitment of reserve cells in human skeletal muscle.Insulin-like growth factor 1 and muscle growth: implication for satellite cell proliferation.Machida S, Booth FW.Department of Biomedical Sciences, University of Missouri-Columbia, E102 Veterinary Medical Building, 1600 East Rollins Road, Columbia, MO 65211, USA.Insulin-like growth factor 1 (IGF-1) has been shown to rescue the aging-related or inactivity-induced loss of muscle mass through the activation of satellite cells. However, the signalling pathways and the mechanism by which IGF-1 affects satellite cells have not been not completely identified. The purpose of the present review is to provide current understanding of the cellular and molecular events underlying IGF-1 induced proliferation of satellite cells.Expression and splicing of the insulin-like growth factor gene in rodent muscle is associated with muscle satellite (stem) cell activation following local tissue damage.Hill M, Goldspink G.Basic Medical Sciences and Department of Surgery, Royal Free and University College Medical School, Royal Free Campus, Rowland Hill Street, London NW3 2PF, UK.Muscle satellite cells are mononuclear cells that remain in a quiescent state until activated when they proliferate and fuse with muscle fibres to donate nuclei, a process necessary for post-embryonic growth, hypertrophy and tissue repair in this post-mitotic tissue. These processes have been associated with expression of the insulin-like growth factor (IGF-I) gene that can undergo alternative splicing to generate different gene products with varying functions. To gain insight into the cellular mechanisms involved in local tissue repair, the time courses of expression of two IGF-I splice variants produced in muscle were determined together with marker genes for satellite cell activation following local muscle damage. Using real-time RT-PCR with specific primers, the mRNA transcripts in rat tibialis anterior muscles were measured at different time intervals following either mechanical damage imposed by electrical stimulation of the stretched muscle or damage caused by injection with bupivacaine. It was found that the autocrine splice variant mechano growth factor (MGF) was rapidly expressed and then declined within a few days following both types of damage. Systemic IGF-IEa was more slowly upregulated and its increase was commensurate with the rate of decline in MGF expression. Satellite cell activation as measured by M-cadherin and one of the muscle regulatory factors MyoD and the sequence of expression suggests that the initial pulse of MGF is responsible for satellite cell activation, as the systemic IGF-IEa mRNA expression peaks after the expression of these markers, including M-cadherin protein. Later splicing of the IGF-I gene away from MGF but towards IGF-IEa seems physiologically appropriate as IGF-IEa is the main source of mature IGF-I for upregulation of protein synthesis required to complete the repair.GH on Muscle:Growth hormone / insulin-like growth factor-1 axis during puberty.Christoforidis A, Maniadaki I, Stanhope R.Department of Endocrinology,Great Ormond Street Hospital and the Middlesex Hospital (UCLH), London, UK.Puberty is a dynamic, transitional period of life which is characterized by the acquisition of secondary sexual characteristics leading to the development of fertility. Puberty is accompanied by sexually dimorphic changes in linear growth, body proportions and body composition. The pubertal growth spurt is influenced by a number of factors such as hormones, nutrition, physical activity and general health, acting mostly in concert in order to modify a genetic potential for growth. Growth hormone (GH) is traditionally considered to be the main regulator of growth. During puberty, elevated sex steroid concentrations (especially oestrogens) stimulate GH production, leading to an activation of the whole GH/Insulinlike growth factor-1 (IGF-1) axis. This activation is mostly characterized by an increase in the amplitude of GH pulses rather than an increase in frequency or in duration. Interactions between GH and sex steroids (especially androgens) express an anabolic effect on muscle mass, bone mineralization and body proportion which constitutes the male and the female adult body composition.Intact insulin and insulin-like growth factor-I receptor signaling is required for growth hormone effects on skeletal muscle growth and function in vivo.Kim H, Barton E, Muja N, Yakar S, Pennisi P, Leroith D.Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Building 10, Room 8D12, Bethesda, Maryland 20892-1758, USA.GH and IGF-I are potent regulators of muscle growth and function. Although IGF-I is known to mediate many of the effects of GH, it is not yet clear whether all effects of GH are completely dependent on the IGF-I system. To evaluate the biological effects of the GH/IGF-I axis on muscle growth, we administrated recombinant human GH to mice, which lack IGF-I function specifically in skeletal muscle, due to the overexpression of a dominant-negative IGF-I receptor in this tissue (MKR mice). GH treatment significantly increased the levels of hepatic IGF-I mRNA and serum IGF-I levels in both wild-type (WT) and MKR mice. These GH-induced effects were paralleled by increases in body weight and in the weights of most GH-responsive organs in both groups of mice. Interestingly, unlike WT mice, GH treatment had no effect on skeletal muscle weight in MKR mice. GH treatment failed to reverse the impaired muscle function in MKR mice. Furthermore, MKR mice exhibited no effects of GH on the cross-sectional area of myofibers and the proliferation of satellite cells. Taken together, these data suggest that the in vivo effects of GH on muscle mass and strength are primarily mediated by activation of the IGF-I receptor.Insulin-like growth factor 1 and muscle growth: implication for satellite cell proliferation.Machida S, Booth FW.Department of Biomedical Sciences, University of Missouri-Columbia, E102 Veterinary Medical Building, 1600 East Rollins Road, Columbia, MO 65211, USA.Insulin-like growth factor 1 (IGF-1) has been shown to rescue the aging-related or inactivity-induced loss of muscle mass through the activation of satellite cells. However, the signalling pathways and the mechanism by which IGF-1 affects satellite cells have not been not completely identified. The purpose of the present review is to provide current understanding of the cellular and molecular events underlying IGF-1 induced proliferation of satellite cells.The effects of growth hormone and/or testosterone in healthy elderly men: a randomized controlled trial.Giannoulis MG, Sonksen PH, Umpleby M, Breen L, Pentecost C, Whyte M, McMillan CV, Bradley C, Martin FC.Department of Diabetes and Endocrinology, GKT School of Medicine, King's College London, St. Thomas' Hospital, London SE1 7EH, UK.CONTEXT:Declines in GH and testosterone (Te) secretion may contribute to the detrimental aging changes of elderly men. OBJECTIVE: To assess the effects of near-physiological GH with/without Te administration on lean body mass, total body fat, midthigh muscle cross-section area, muscle strength, aerobic capacity, condition-specific quality of life (Age-Related Hormone Deficiency-Dependent Quality of Life questionnaire), and generic health status (36-Item Short-Form Health Survey) of older men. DESIGN, SETTINGS, AND PARTICIPANTS: A 6-month, randomized, double-blind, placebo-controlled trial was performed on 80 healthy, community-dwelling, older men (age, 65-80 yr). INTERVENTIONS: Participants were randomized to receive 1) placebo GH or placebo Te, 2) recombinant human GH (rhGH) and placebo Te (GH), 3) Te and placebo rhGH (Te), or 4) rhGH and Te (GHTe). GH doses were titrated over 8 wk to produce IGF-I levels in the upper half of the age-specific reference range. A fixed dose of Te (5 mg) was given by transdermal patches. RESULTS: Lean body mass increased with GHTe (P = 0.008) and GH (P = 0.004), compared with placebo. Total body fat decreased with GHTe only (P = 0.02). Midthigh muscle (P = 0.006) and aerobic capacity (P < p =" 0.007)" p =" 0.003)." href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstract&term=">Ehrnborg C, Ellegard L, Bosaeus I, Bengtsson BA, Rosen T.Research Centre for Endocrinology and Metabolism, Department of Internal Medicine, Sahlgrenska University Hospital, S-413 45 Goteborg, Sweden. christer.ehrnborg@medic.gu.seOBJECTIVES: To study the effects on body composition after 1 month's administration of supraphysiological doses of growth hormone (GH) in healthy, active young adults with normal GH-IGF-I axis. SUBJECTS AND METHODS: Thirty healthy, physically active volunteers (15 men and 15 women), mean age 25.9 years (range 18-35), participated in this study, designed as a randomized, double-blind, placebo-controlled, parallel study with three groups (n = 10: five men and five women in each group). The groups comprised the following: placebo (P), GH 0.1 IU/kg/day [0.033 mg/kg/day] (GH 0.1) and GH 0.2 IU/kg/day [0.067 mg/kg/day] (GH 0.2). RESULTS: In the pooled group with active GH treatment (n = 20) the results showed significant increases: IGF-I increased by 134% (baseline vs. after 1 month), body weight by 2.7%, fat free mass by 5.3%, total body water by 6.5% and extracellular water (ECW) by 9.6%. Body fat decreased significantly by 6.6%. No significant change in intracellular water was detected. The observed increase in fat free mass by 5.3% was explained by the ECW increase, indicating limited anabolic effects of the supraphysiological GH doses. Changes were noticeable in both genders, although more prominent in the male subjects. Fluid retention symptoms occurred in the majority of individuals. CONCLUSIONS: This is, to our knowledge, the first placebo-controlled trial to show the effects of supraphysiological GH doses on body composition and IGF-I levels in physically active and healthy individuals of both genders; the results indicate limited anabolic effects of GH with these supraphysiological doses. The role of GH as an effective anabolic-doping agent is questioned.GH alone is not enough, which is why we stack…Here is an example of an effective stacking of compatible supplements to achieve a specific objective.Regulating of growth hormone sensitivity by sex steroids: implications for therapy.KK, Gibney J, Johannsson G, Wolthers T.Pituitary Research Unit, Garvan Institute of Medical Research and Department of Endocrinology, St. Vincent's Hospital, Sydney, Australia.Growth hormone (GH) is an important regulator of body composition, reducing body fat by stimulating fat oxidation and enhancing lean body mass by stimulating protein accretion. The emergence of differences in body composition between the sexes during puberty suggests sex steroids modulate the action of GH. Work from our laboratory have investigated the influence of estrogens and androgens on the metabolic actions of GH in human adults. The liver is an important site of physiological interaction as it is a sex steroid responsive organ and a major target of GH action. Estrogen, when administered orally impairs the GHregulated endocrine and metabolic function of the liver via a first-pass effect. It reduces circulating IGF-I, fat oxidation and protein synthesis, contributing to a loss of lean and a gain of fat mass. These effects occur in normal and in GH-deficient women and are avoided by transdermal administration of physiological doses of estrogen. In contrast, studies in hypopituitary men indicate that testosterone enhances the metabolic effects of GH. Testosterone alone stimulates fat oxidation and protein synthesis, both of which are enhanced by GH. Studies in GH deficiency adults have consistently reported women to be less sensitive to GH than men. In summary, estrogens and androgens exert divergent effects on the action of GH. The results provide an explanation for sexual dimorphism in body composition in adults and the genderrelated response to GH replacement in hypopituitary subjects. In the management of hypopituitarism, estrogens should be administered by the parenteral route in women and testosterone be replaced in men to optimize the benefits of GH replacement.Eric D. Marchewitz, is one of the leading supplement experts in the country, his articles online are taken from his best selling book " Supplements For Genetic Growth " which explains how you can increase the number of muscle cells in your body using supplement stacks available at any health food store. This book will demonstrate how the body will try and resist your efforts to grow insane muscles and how, as you age, the ability to create new muscle cells decline so make those cells now! The book is backed by science! This is the most amazing break through in supplement history! The book is available from the LG Sciences website http://www.lgsciences.com/
NOS Precursors and Satellite Cells
NO is a potent vasodilator which means that it causes blood vessels to open up allowing more blood to pass through. This results in increased blood being delivered to your muscles resulting in swelling of the muscles known as "The Pump". This swelling not only looks impressive but also results in the same kind of stretching as Creatine, resulting in increased protein synthesis. Also, the increased blood flow means more oxygen and more nutrients flowing into muscles where they can be used to provide energy and support hypertrophy while more toxins and waste products are removed from the muscles.Citrulline can be made from arginine as explained above, but it is also possible to make arginine from citrulline. Therefore, increasing citrulline creates an additional pool of arginine for the body to use as a precursor to NO (Nitric Oxide).Everyone has heard about Nitric Oxide precursors and their effects on vasodilatation (opening of the veins and arteries to increase blood flow), but you may not have heard about how NO precursors like Arginine and its various forms along with things like Citrulline Malate and other potentiators of NO can greatly increase the chances of muscle cell differentiation.Nitric Oxide is produced in the bloodstream as a mechanism of dilating blood vessels, but in the cell it is used to increase the transformation of satellite cells into mature muscle cells, this further increasing your ability to transform your genetic potential. Arginine is an amino acid that is often bound to a carrier or delivery molecule like malic acid, alpha-keto-glutarate or orotic acid to increase its potency and bioavailability. L-Citrulline is an amino acid that will further increase blood levels of Arginine and further increase your ability to make new muscle cells just waiting to be filled with protein. The other interesting thing about NO is that it is released by muscles during a workout to help pump more blood into your working muscles. Using a combination of topical and systemic (full body) Arginine-AKG products can vastly increase the amount of NO released in your muscle tissue. That’s why Arginine products have such a great reputation among bodybuilders for increasing size and vascularity. Another big benefit is that Arginine helps burn stored body fat by up regulating the use of fats for fuel. So Arginine has a double benefit of increasing size and strength while cutting fat.NITROS™, LG Sciences has both Arginine-AKG and Citrulline Malate along with a host of other NO boosting agents. It’s the most advanced product on the market today and makes for a great part of your genetic stack to boost NO and build muscle using a systemic (full body) approach. There is another way to deliver NO boosting nutrient where they are needed most that is topically through the use of Arginine. You might have a hard time believing this, but topical products deliver nutrients better in some cases than oral products and increase the amount of NO in a specific muscle group. There are quite a few products on the market, but my favorite is obviously NO INFUSE™ by LG Sciences.NO INFUSE™ is the best topical product I have ever used. It makes my veins explode and warms me up before every workout. The other products I have tried literally burn your skin with painful ingredients. I had to wash off one of the products because it stung so badly. It has been suggested that one could use a similar product to increase blood flow to the penis. However, it hurt so bad on my arms that I wouldn’t be using it anywhere close to my manhood. LG Sciences NO INFUSE™ doesn’t have this issue, as it is completely painless and about the only side effect I noticed is a slight drying of my skin, which I remedied by using lotion later in the day. LG Sciences NO INFUSE™ can also boost the satellite expression directly into your trouble areas by targeting NO release where you need it most.LG Sciences NO Infuse is specially formulated to penetrate the skin deep into the tissue with its amazing delivery system. Once through the skin, the unique NO releasing array of ingredients goes directly to your muscles to stimulate nitric oxide production resulting in improved blood flow, mind-blowing muscle pumps and satellite expression.An issue with NO products is that you shouldn’t take them with high doses of caffeine and other stimulants that can naturally constrict the blood vessels, since you will be countering all of the benefits that NO production can bring. Also, take the Arginine oral products on an empty stomach and use the topical Arginine as a pre-workout warm up rub and then once again after the workout to keep the blood flowing to your freshly pumped muscles.Below is an abstract showing Arginine and NO increasing satellite cell proliferation and expression. There are others that show how Arginine decreases fat, and this should help you see how you can gain valuable synergy when stacking items in this book. It’s really amazing that we have so many good products on the market to help you reach your goal of genetic transformation.Systemic administration of L-arginine benefits mdx skeletal muscle function.Barton ER, Morris L, Kawana M, Bish LT, Toursel T.Department of Anatomy and Cell Biology, School of Dental Medicine, 441 Levy Building, 240 South 40th Street, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. erbarton@biochem.dental.upenn.eduA major consequence of muscular dystrophy is that increased membrane fragility leads to high calcium influx and results in muscle degeneration and myonecrosis. Prior reports have demonstrated that increased nitric oxide production via L-arginine treatment of normal and mdx mice resulted in increased expression of utrophin and increased activation of muscle satellite cells, which could ameliorate the dystrophic pathology. We delivered L-arginine to normal and mdx mice, and examined muscles for any functional changes associated with its administration. Treated mdx muscles were less susceptible to contraction-induced damage and exhibited a rightward shift of the force-frequency relationship. Immunoblotting revealed increases in utrophin and gamma-sarcoglycan in the treated muscles. There was also a decrease in Evans blue dye uptake, indicating a reduction in myonecrosis. However, there was no decrease in serum creatine kinase or the proportion of central nuclei, nor any improvement in specific force. Together, these results show that L-arginine treatment can be beneficial to mdx muscle function, perhaps through a combination of enhanced calcium handling and increased utrophin, thereby decreasing muscle degeneration.Eric D. Marchewitz, is one of the leading supplement experts in the country, his articles online are taken from his best selling book " Supplements For Genetic Growth " which explains how you can increase the number of muscle cells in your body using supplement stacks available at any health food store. This book will demonstrate how the body will try and resist your efforts to grow insane muscles and how, as you age, the ability to create new muscle cells decline so make those cells now! The book is backed by science! This is the most amazing break through in supplement history! The book is available from the LG Sciences website www.lgsciences.com
NO is a potent vasodilator which means that it causes blood vessels to open up allowing more blood to pass through. This results in increased blood being delivered to your muscles resulting in swelling of the muscles known as "The Pump". This swelling not only looks impressive but also results in the same kind of stretching as Creatine, resulting in increased protein synthesis. Also, the increased blood flow means more oxygen and more nutrients flowing into muscles where they can be used to provide energy and support hypertrophy while more toxins and waste products are removed from the muscles.Citrulline can be made from arginine as explained above, but it is also possible to make arginine from citrulline. Therefore, increasing citrulline creates an additional pool of arginine for the body to use as a precursor to NO (Nitric Oxide).Everyone has heard about Nitric Oxide precursors and their effects on vasodilatation (opening of the veins and arteries to increase blood flow), but you may not have heard about how NO precursors like Arginine and its various forms along with things like Citrulline Malate and other potentiators of NO can greatly increase the chances of muscle cell differentiation.Nitric Oxide is produced in the bloodstream as a mechanism of dilating blood vessels, but in the cell it is used to increase the transformation of satellite cells into mature muscle cells, this further increasing your ability to transform your genetic potential. Arginine is an amino acid that is often bound to a carrier or delivery molecule like malic acid, alpha-keto-glutarate or orotic acid to increase its potency and bioavailability. L-Citrulline is an amino acid that will further increase blood levels of Arginine and further increase your ability to make new muscle cells just waiting to be filled with protein. The other interesting thing about NO is that it is released by muscles during a workout to help pump more blood into your working muscles. Using a combination of topical and systemic (full body) Arginine-AKG products can vastly increase the amount of NO released in your muscle tissue. That’s why Arginine products have such a great reputation among bodybuilders for increasing size and vascularity. Another big benefit is that Arginine helps burn stored body fat by up regulating the use of fats for fuel. So Arginine has a double benefit of increasing size and strength while cutting fat.NITROS™, LG Sciences has both Arginine-AKG and Citrulline Malate along with a host of other NO boosting agents. It’s the most advanced product on the market today and makes for a great part of your genetic stack to boost NO and build muscle using a systemic (full body) approach. There is another way to deliver NO boosting nutrient where they are needed most that is topically through the use of Arginine. You might have a hard time believing this, but topical products deliver nutrients better in some cases than oral products and increase the amount of NO in a specific muscle group. There are quite a few products on the market, but my favorite is obviously NO INFUSE™ by LG Sciences.NO INFUSE™ is the best topical product I have ever used. It makes my veins explode and warms me up before every workout. The other products I have tried literally burn your skin with painful ingredients. I had to wash off one of the products because it stung so badly. It has been suggested that one could use a similar product to increase blood flow to the penis. However, it hurt so bad on my arms that I wouldn’t be using it anywhere close to my manhood. LG Sciences NO INFUSE™ doesn’t have this issue, as it is completely painless and about the only side effect I noticed is a slight drying of my skin, which I remedied by using lotion later in the day. LG Sciences NO INFUSE™ can also boost the satellite expression directly into your trouble areas by targeting NO release where you need it most.LG Sciences NO Infuse is specially formulated to penetrate the skin deep into the tissue with its amazing delivery system. Once through the skin, the unique NO releasing array of ingredients goes directly to your muscles to stimulate nitric oxide production resulting in improved blood flow, mind-blowing muscle pumps and satellite expression.An issue with NO products is that you shouldn’t take them with high doses of caffeine and other stimulants that can naturally constrict the blood vessels, since you will be countering all of the benefits that NO production can bring. Also, take the Arginine oral products on an empty stomach and use the topical Arginine as a pre-workout warm up rub and then once again after the workout to keep the blood flowing to your freshly pumped muscles.Below is an abstract showing Arginine and NO increasing satellite cell proliferation and expression. There are others that show how Arginine decreases fat, and this should help you see how you can gain valuable synergy when stacking items in this book. It’s really amazing that we have so many good products on the market to help you reach your goal of genetic transformation.Systemic administration of L-arginine benefits mdx skeletal muscle function.Barton ER, Morris L, Kawana M, Bish LT, Toursel T.Department of Anatomy and Cell Biology, School of Dental Medicine, 441 Levy Building, 240 South 40th Street, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. erbarton@biochem.dental.upenn.eduA major consequence of muscular dystrophy is that increased membrane fragility leads to high calcium influx and results in muscle degeneration and myonecrosis. Prior reports have demonstrated that increased nitric oxide production via L-arginine treatment of normal and mdx mice resulted in increased expression of utrophin and increased activation of muscle satellite cells, which could ameliorate the dystrophic pathology. We delivered L-arginine to normal and mdx mice, and examined muscles for any functional changes associated with its administration. Treated mdx muscles were less susceptible to contraction-induced damage and exhibited a rightward shift of the force-frequency relationship. Immunoblotting revealed increases in utrophin and gamma-sarcoglycan in the treated muscles. There was also a decrease in Evans blue dye uptake, indicating a reduction in myonecrosis. However, there was no decrease in serum creatine kinase or the proportion of central nuclei, nor any improvement in specific force. Together, these results show that L-arginine treatment can be beneficial to mdx muscle function, perhaps through a combination of enhanced calcium handling and increased utrophin, thereby decreasing muscle degeneration.Eric D. Marchewitz, is one of the leading supplement experts in the country, his articles online are taken from his best selling book " Supplements For Genetic Growth " which explains how you can increase the number of muscle cells in your body using supplement stacks available at any health food store. This book will demonstrate how the body will try and resist your efforts to grow insane muscles and how, as you age, the ability to create new muscle cells decline so make those cells now! The book is backed by science! This is the most amazing break through in supplement history! The book is available from the LG Sciences website www.lgsciences.com
Methyl 1-D
METHYL 1-D™The Prohormone that slipped past the ban – Get it while you can!You know that prohormones give you explosive muscle gains and skyrocketing strength that surpasses your wildest dreams. Except you can’t get them now…or can you? With every prohormone we’ve created, we look for those compounds that do an awesome job of building muscles but don’t cross that legal line.It’s hard to stay right here, where we always have been, on the cutting edge of supplement enhancement. Legal Gear (or should we say LG Sciences) has ALWAYS led the PROHORMONE revolution. We started with Methyl 1-Test, a legal steroid that had bodybuilders screaming in the streets. We followed up with products like Masterdrol V2, Methyl 1-Alpha, Methyl Masterdrol and the favorite of all time, Methyl 1-P.Despite the ban, we’ve still found a way to make muscle magic with the ONLY real PROHORMONE still available today – METHYL 1-D. Don’t be fooled by “hormone-like”, “similar to prohormone,” “steroid alternative,” or any of the other bogus terms used for products that aren’t really prohormones. Methyl 1-D is the real thing, and it does everything you expect from a prohormone despite the ban.METHYL 1-D™, is our most groundbreaking product yet. Only METHYL 1-D™ is an anabolic animal by itself AND a stack supercharger, too!The Last Legal Prohormone Product AvailableMETHYL 1-D™ may be one of the most potent androgens since the prohormone ban in January 2005. With METHYL-1D, you get an AAS (anabolic/androgenic steroid) hormone that’s a chemical cousin to Testosterone. METHYL 1-D converts easily into TEST and also has an estrogen blocker to prevent bloat and bitch tits along with a 5aR inhibitor, so you can keep your hair. This is by far the safest product on the market that still gives prohormone results. You’ll get solid gains in muscle mass without the bloated look you get from many steroids.Additionally, you may also see yourself become more cut with every week because one pleasant side effect may be the blocking of an enzyme called glucose-6-phosphate-dehydrogenase. Scientists believe this enzyme may block the body’s ability to store and produce fat. One study showed a mean body fat reduction of 31% in only 28 days – with no change in body weight – which means more muscle and less fat! I personally saw this first hand on my friend that took Methyl-1D for 3 weeks and lost 3 inches on his waist while his bench press went up 30 lbs!Did we forget to mention the effects on libido and sexual endurance? Study after study shows how the compounds in METHYL 1-D improve every aspect of your sexual performance too.You will feel Methyl-1D immediately because it gives instant results that you can count on time and time again. Studies with the ingredients in METHYL 1-D show improvements within two week in strength, mass and body fat ratio but you’ll begin to feel the effects with your very first dose. Potential benefits of Methyl 1-D are:Extreme Gains in Strength and SizeSkyrocketing Testosterone LevelsIncreased Muscle MassSupercharged Sex DriveIncreased Fat LossNo Gyno Or Estrogen Effects – due to the estrogen blockerNo Hair Loss – because we block DHTRamped up Metabolism and ThermogenesisGreater Conversion of Glucose to Energy rather than Fat.Incredible Sense of Power That Comes from a Boost in TestosteroneUnlike other supplements out there, you’ll get the full benefit of all the ingredients in METHYL 1-D. It’s as potent as any methyl on the market today because of a patent pending special delivery mechanism.METHYL 1-D Supercharges Our Other Products for the Most Powerful ResultsIf you’re ready for some radical changes in your body, combine METHYL 1-D™ with our other products. Methyl 1-D synergisticly supercharges the effects of our other products so that you get more of what you buy supplements for – muscle mass, strength, fat burning, etc. . Each LG Science product is specifically designed to work with METHYL 1-D™ as part of a cycle for truly astounding results.To MELT FAT – Combine METHYL 1-D with SPEED. You’ll supercharge your metabolism for a fat melting bonfire with this prohormone/stimulant combination.To radically CHANGE YOUR BODY COMPOSITION AND BLOCK CORTISOL. TRANSFORM your body when you combine METHYL 1-D with TRANSFORM. This combination encourages muscle growth while preventing muscle catabolism and cortisol damage.The NITRIC OXIDE power of NITROS with METHYL 1-D feeds those muscles that you create like crazy. Fill ‘em up, pump ‘em up and feed them right with increased vascularity, blood flow and nutrient delivery to your muscle cells.For POST CYCLE RECOVERY and to BOOST and MAINTAIN TESTOSTERONE LEVELS after METHYL 1-D, use Formadrol Extreme. Increase you testosterone naturally, block estrogen conversion and increase IGF-1 levels so you can keep 100% of your gains!LG Sciences gives you the ultimate in GENETIC GROWTH. When you combine all of our products, we will show you how you can CHANGE YOUR MUSCLE DENSITY FOREVER! We give you the science behind every product to show how you can increase satellite cell growth. With Methyl 1-D, we can show how androgens create satellite cells to change your genetics and increase hypertrophy. First we need to define how you grow so you understand:Hypertrophy – building your existing muscle cells (filling them up with protein), this is why you plateau and only grow so muchHyperplasia – building NEW muscle cells via satellite cells, this is how you get new muscle growth and push past any genetic plateau. Each supplement contains the ingredients to cause hypertrophy and hyperplasia and can be combined for even greater growth backed by science:Androgen receptor in human skeletal muscle and cultured muscle satellite cells: up-regulation by androgen treatment.Sinha-Hikim I, Taylor WE, Gonzalez-Cadavid NF, Zheng W, Bhasin S.Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA.“These observations support the hypothesis that androgens increase muscle mass in part by acting on several cell types to regulate the differentiation of mesenchymal precursor cells in the skeletal muscle.”
METHYL 1-D™The Prohormone that slipped past the ban – Get it while you can!You know that prohormones give you explosive muscle gains and skyrocketing strength that surpasses your wildest dreams. Except you can’t get them now…or can you? With every prohormone we’ve created, we look for those compounds that do an awesome job of building muscles but don’t cross that legal line.It’s hard to stay right here, where we always have been, on the cutting edge of supplement enhancement. Legal Gear (or should we say LG Sciences) has ALWAYS led the PROHORMONE revolution. We started with Methyl 1-Test, a legal steroid that had bodybuilders screaming in the streets. We followed up with products like Masterdrol V2, Methyl 1-Alpha, Methyl Masterdrol and the favorite of all time, Methyl 1-P.Despite the ban, we’ve still found a way to make muscle magic with the ONLY real PROHORMONE still available today – METHYL 1-D. Don’t be fooled by “hormone-like”, “similar to prohormone,” “steroid alternative,” or any of the other bogus terms used for products that aren’t really prohormones. Methyl 1-D is the real thing, and it does everything you expect from a prohormone despite the ban.METHYL 1-D™, is our most groundbreaking product yet. Only METHYL 1-D™ is an anabolic animal by itself AND a stack supercharger, too!The Last Legal Prohormone Product AvailableMETHYL 1-D™ may be one of the most potent androgens since the prohormone ban in January 2005. With METHYL-1D, you get an AAS (anabolic/androgenic steroid) hormone that’s a chemical cousin to Testosterone. METHYL 1-D converts easily into TEST and also has an estrogen blocker to prevent bloat and bitch tits along with a 5aR inhibitor, so you can keep your hair. This is by far the safest product on the market that still gives prohormone results. You’ll get solid gains in muscle mass without the bloated look you get from many steroids.Additionally, you may also see yourself become more cut with every week because one pleasant side effect may be the blocking of an enzyme called glucose-6-phosphate-dehydrogenase. Scientists believe this enzyme may block the body’s ability to store and produce fat. One study showed a mean body fat reduction of 31% in only 28 days – with no change in body weight – which means more muscle and less fat! I personally saw this first hand on my friend that took Methyl-1D for 3 weeks and lost 3 inches on his waist while his bench press went up 30 lbs!Did we forget to mention the effects on libido and sexual endurance? Study after study shows how the compounds in METHYL 1-D improve every aspect of your sexual performance too.You will feel Methyl-1D immediately because it gives instant results that you can count on time and time again. Studies with the ingredients in METHYL 1-D show improvements within two week in strength, mass and body fat ratio but you’ll begin to feel the effects with your very first dose. Potential benefits of Methyl 1-D are:Extreme Gains in Strength and SizeSkyrocketing Testosterone LevelsIncreased Muscle MassSupercharged Sex DriveIncreased Fat LossNo Gyno Or Estrogen Effects – due to the estrogen blockerNo Hair Loss – because we block DHTRamped up Metabolism and ThermogenesisGreater Conversion of Glucose to Energy rather than Fat.Incredible Sense of Power That Comes from a Boost in TestosteroneUnlike other supplements out there, you’ll get the full benefit of all the ingredients in METHYL 1-D. It’s as potent as any methyl on the market today because of a patent pending special delivery mechanism.METHYL 1-D Supercharges Our Other Products for the Most Powerful ResultsIf you’re ready for some radical changes in your body, combine METHYL 1-D™ with our other products. Methyl 1-D synergisticly supercharges the effects of our other products so that you get more of what you buy supplements for – muscle mass, strength, fat burning, etc. . Each LG Science product is specifically designed to work with METHYL 1-D™ as part of a cycle for truly astounding results.To MELT FAT – Combine METHYL 1-D with SPEED. You’ll supercharge your metabolism for a fat melting bonfire with this prohormone/stimulant combination.To radically CHANGE YOUR BODY COMPOSITION AND BLOCK CORTISOL. TRANSFORM your body when you combine METHYL 1-D with TRANSFORM. This combination encourages muscle growth while preventing muscle catabolism and cortisol damage.The NITRIC OXIDE power of NITROS with METHYL 1-D feeds those muscles that you create like crazy. Fill ‘em up, pump ‘em up and feed them right with increased vascularity, blood flow and nutrient delivery to your muscle cells.For POST CYCLE RECOVERY and to BOOST and MAINTAIN TESTOSTERONE LEVELS after METHYL 1-D, use Formadrol Extreme. Increase you testosterone naturally, block estrogen conversion and increase IGF-1 levels so you can keep 100% of your gains!LG Sciences gives you the ultimate in GENETIC GROWTH. When you combine all of our products, we will show you how you can CHANGE YOUR MUSCLE DENSITY FOREVER! We give you the science behind every product to show how you can increase satellite cell growth. With Methyl 1-D, we can show how androgens create satellite cells to change your genetics and increase hypertrophy. First we need to define how you grow so you understand:Hypertrophy – building your existing muscle cells (filling them up with protein), this is why you plateau and only grow so muchHyperplasia – building NEW muscle cells via satellite cells, this is how you get new muscle growth and push past any genetic plateau. Each supplement contains the ingredients to cause hypertrophy and hyperplasia and can be combined for even greater growth backed by science:Androgen receptor in human skeletal muscle and cultured muscle satellite cells: up-regulation by androgen treatment.Sinha-Hikim I, Taylor WE, Gonzalez-Cadavid NF, Zheng W, Bhasin S.Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California 90059, USA.“These observations support the hypothesis that androgens increase muscle mass in part by acting on several cell types to regulate the differentiation of mesenchymal precursor cells in the skeletal muscle.”
Cold Fusion EX: Best Creatine On The Market
Cold Fusion EX increases strength, stamina and size. We do this by combining a unique ratio of ingredients that are proven in the scientific studies to perform a unique function. These combined are why Cold Fusion is up to SIX times better than standard creatine. This article is heavy on the science, but rest assured that this means that you are getting the best quality product with the heavy science to back it up. Seth (my scientist) wrote the technical stuff, I will translate for us “less science minded” Want a free sample bottle? Visit www.lgsciences.com
Creatine Ethyl Ester: Creatine ethyl ester is one of the newest forms of creatine. The ethyl ester prevents breakdown in the gut and prolongs the life of creatine in the blood. Combined with creatine monohydrate this results in a one-two punch of fast acting immediate action coupled with the longer duration of creatine ethyl ester. – CEE gives you a longer acting creatine, the right amount works, too much and it tastes like battery acid.
Creatine Monohydrate: The granddaddy of all creatine. Creatine monohydrate has been proven time and time again in the scientific literature to not only increase stamina and strength but also to cause skeletal muscle growth through hypertrophy (expansion of existing muscle cells) and hyperplasia (the creation of new muscle cells from stem cells). Creatine loads water and nutrients into muscle cells resulting in massive pumps and an enhanced anabolic state. – Nothing works like Creatine Monohydrate, it is proven and is shown to increase the forming of new muscle cells. We use 200mesh pharmaceutical grade.
Creatine Malate: Creatine bonded to the tricarboxylic acid cycle intermediate, L-Malate. L-Malate plays a critical role in shuttling NADH, an energy molecule, from the cytoplasm into the mitochondria for energy production. This results in an increase in physical stamina and a reduction in skeletal muscle damage (1). – Malate’s increase the energy production of creatine, giving you increased stamina.
Taurine: Taurine is part of the native defense mechanism against oxidative damage in skeletal muscle (2). Taurine is also a modulator of contractile function in mammalian skeletal muscle and increases the force responses by augmenting calcium accumulation and release (3). – Taurine gives you extra energy and pumps.
L-Leucine: Leucine has been shown to not only reduce the rate of protein degradation in skeletal muscle as a result of resistance training but to also increase protein accumulation and strength (4). – Leucine is the first amino acid used up when you work out. It is proven to be anabolic.
Beta Alanine: Beta Alanine is an amino acid that is a component of carnosine. Stuides have shown that beta alanine supercharges the effects of creatine, resulting in greater lean mass gains and strength increases (5). – Beta Alanine buffers lactic acid and gives you longer workout times and reduces failure.
Aspartic Acid: Aspartic acid plays a major role in the malate-aspartate shuttle and acts to recharge L-Malate. Supplementation with aspartic acid has resulted in increased endurance and decreased lactic acid buildup. Furthermore, studies show decreased rate of glycogen depletion during exercise due to increased utilization of free fatty acids over glycogen (6,7) – Aspartic Acid increases endurance and performance.
Yohimbine HCL: Yohimbine is an alpha-adrenergic antagonist. Supplementation with yohimbine results in augmentation of nitric oxide synthesis and increased vasodilation – which means more blood, water and nutrients delivered to your muscles. – Yohimbine gives you the mental lift in the gym, without the crash of caffeine. It also increases blood flow, making NO work better.L-Arginine: L-Arginine is THE precursor to nitric oxide (NO) production. Nitric oxide is the major vasodilator in blood vessels. Vasodilation causes blood vessels to open up, allowing more blood to flow. This gives you the massive pumps that you experience when supplementing with arginine. This pump not only looks great but it also helps to build muscle by delivering more nutrients where you need it most. – Arginine is the base for a good NO pump, we prefer a mix of many types to get the maximum benefit.
Arginine AKG: Arginine bound to the tricarboxylic acid intermediate, alpha-ketoglutate. Alpha-ketoglutarate is important for driving the malate-aspartate shuttle and is one of the most important nitrogen transporters. In addition, alpha-ketoglutarate is a precursor to the neurotransmitters glutamate and GABA. – Arginine AKG is a good form of Arginine proven to help build strength and give pumps.
Arginine Orotate: Arginine bound to orotic acid results in a longer duration of action and improved absorption of arginine. – Another form of Arginine that lasts longer in the body.Mormordica: Momordica is an herbal ingredient that has been used in China for over a thousand years. Momordica upregulates the expression of GLUT-4, the primary glucose transporter in skeletal muscle. This results in increased glucose and protein transport into muscle. This increased flow of nutrients also loads creatine and water into the muscle (8,9). – This stuff is really cool, it shuttles all the other stuff into the muscle. Unlike inferior products (like ALA) it pushes both amino acids and sugar into the muscle (all the above stuff are amino acids, which is why Cold Fusion works better than anything else).
Pomegranate Powder: Pomegranate juice contains potent antioxidants that protect nitric oxide (NO) from oxidative destruction. This results in an increase in the activity of NO as well as the duration of action (10). – Pomegranate is good for you in many ways, but it is shown to increase your pumps by extending the life of NO.
Spirulina: Spirulina has been shown to decrease oxidative damage in skeletal muscle but it also has another interesting effect. Spirulina augments the vasodilating effect of nitric oxide while at the same time, decreasing vasoconstriction by reducing the synthesis and release of vasoconstricting eicosanoids (11). – Spirulina is known as an energy food that increases pumps and reduces muscular fatigue.
This unique combination of ingredients will give you the biggest pumps during your workouts while at the same time producing the optimal anabolic state. Hopefully, our dual explanations give you science guys and normal dudes a chance to see why Cold Fusion EX is so great. Oh, you have to taste it! It is freaking amazing.
ReferencesWu J, Wu Q, Huang J, Chen R, Cai M, Tan J. Effects of L-malate on physical stamina and activities of enzymes related to the malate-aspartate shuttle in liver of mice. Physiol Res. 2006 Mar 23Kingston R, Kearns S, Kelly C, Murray P. Effects of systemic and regional taurine on skeletal muscle function following ischaemia-reperfusion injury. J Orthop Res. Mar;23(2):310-4, 2005Bakker AJ, Berg HM. Effect of taurine on sarcoplasmic reticulum function and force in skinned fast-twitch skeletal muscle fibres of the rat. J Physiol. Jan 1;538(Pt 1):185-94, 2002Coburn JW, Housh DJ, Housh TJ, Malek MH, Beck TW, Cramer JT, Johnson GO, Donlin PE. Effects of leucine and whey protein supplementation during eight weeks of unilateral resistance training. J Strength Cond Res. May;20(2):284-91, 2006Hoffman J, Ratamess N, Kang J, Mangine G, Faigenbaum A, Stout J. Effect of creatine and beta-alanine supplementation on performance and endocrine responses in strength/power athletes. Int J Sport Nutr Exerc Metab. Aug;16(4):430-46, 2006Marquezi ML, Roschel HA, dos Santa Costa A, Sawada LA, Lancha AH Jr. Effect of aspartate and asparagine supplementation on fatigue determinants in intense exercise. Int J Sport Nutr Exerc Metab. Mar;13(1):65-75, 2003Lancha AH Jr, Recco MB, Abdalla DS, Curi R. Effect of aspartate, asparagine, and carnitine supplementation in the diet on metabolism of skeletal muscle during a moderate exercise. Physiol Behav. Feb;57(2):367-71, 1995Cummings E, Hundal HS, Wackerhage H, Hope M, Belle M, Adeghate E, Singh J. Momordica charantia fruit juice stimulates glucose and amino acid uptakes in L6 myotubes. Mol Cell Biochem. Jun;261(1-2):99-104, 2004McCarty MF. Does bitter melon contain an activator of AMP-activated kinase? Med Hypotheses. 63(2):340-3, 2004Ignarro LJ, Byrns RE, Sumi D, de Nigris F, Napoli C. Pomegranate juice protects nitric oxide against oxidative destruction and enhances the biological actions of nitric oxide. Nitric Oxide. Sep;15(2):93-102, 2006Paredes-Carbajal MC, Torres-Duran PV, Diaz-Zagoya JC, Mascher D, Juarez-Oropeza MA. Effects of dietary Spirulina maxima on endothelium dependent vasomotor responses of rat aortic rings. Life Sci. 61(15):PL 211-9, 1997
Cold Fusion EX increases strength, stamina and size. We do this by combining a unique ratio of ingredients that are proven in the scientific studies to perform a unique function. These combined are why Cold Fusion is up to SIX times better than standard creatine. This article is heavy on the science, but rest assured that this means that you are getting the best quality product with the heavy science to back it up. Seth (my scientist) wrote the technical stuff, I will translate for us “less science minded” Want a free sample bottle? Visit www.lgsciences.com
Creatine Ethyl Ester: Creatine ethyl ester is one of the newest forms of creatine. The ethyl ester prevents breakdown in the gut and prolongs the life of creatine in the blood. Combined with creatine monohydrate this results in a one-two punch of fast acting immediate action coupled with the longer duration of creatine ethyl ester. – CEE gives you a longer acting creatine, the right amount works, too much and it tastes like battery acid.
Creatine Monohydrate: The granddaddy of all creatine. Creatine monohydrate has been proven time and time again in the scientific literature to not only increase stamina and strength but also to cause skeletal muscle growth through hypertrophy (expansion of existing muscle cells) and hyperplasia (the creation of new muscle cells from stem cells). Creatine loads water and nutrients into muscle cells resulting in massive pumps and an enhanced anabolic state. – Nothing works like Creatine Monohydrate, it is proven and is shown to increase the forming of new muscle cells. We use 200mesh pharmaceutical grade.
Creatine Malate: Creatine bonded to the tricarboxylic acid cycle intermediate, L-Malate. L-Malate plays a critical role in shuttling NADH, an energy molecule, from the cytoplasm into the mitochondria for energy production. This results in an increase in physical stamina and a reduction in skeletal muscle damage (1). – Malate’s increase the energy production of creatine, giving you increased stamina.
Taurine: Taurine is part of the native defense mechanism against oxidative damage in skeletal muscle (2). Taurine is also a modulator of contractile function in mammalian skeletal muscle and increases the force responses by augmenting calcium accumulation and release (3). – Taurine gives you extra energy and pumps.
L-Leucine: Leucine has been shown to not only reduce the rate of protein degradation in skeletal muscle as a result of resistance training but to also increase protein accumulation and strength (4). – Leucine is the first amino acid used up when you work out. It is proven to be anabolic.
Beta Alanine: Beta Alanine is an amino acid that is a component of carnosine. Stuides have shown that beta alanine supercharges the effects of creatine, resulting in greater lean mass gains and strength increases (5). – Beta Alanine buffers lactic acid and gives you longer workout times and reduces failure.
Aspartic Acid: Aspartic acid plays a major role in the malate-aspartate shuttle and acts to recharge L-Malate. Supplementation with aspartic acid has resulted in increased endurance and decreased lactic acid buildup. Furthermore, studies show decreased rate of glycogen depletion during exercise due to increased utilization of free fatty acids over glycogen (6,7) – Aspartic Acid increases endurance and performance.
Yohimbine HCL: Yohimbine is an alpha-adrenergic antagonist. Supplementation with yohimbine results in augmentation of nitric oxide synthesis and increased vasodilation – which means more blood, water and nutrients delivered to your muscles. – Yohimbine gives you the mental lift in the gym, without the crash of caffeine. It also increases blood flow, making NO work better.L-Arginine: L-Arginine is THE precursor to nitric oxide (NO) production. Nitric oxide is the major vasodilator in blood vessels. Vasodilation causes blood vessels to open up, allowing more blood to flow. This gives you the massive pumps that you experience when supplementing with arginine. This pump not only looks great but it also helps to build muscle by delivering more nutrients where you need it most. – Arginine is the base for a good NO pump, we prefer a mix of many types to get the maximum benefit.
Arginine AKG: Arginine bound to the tricarboxylic acid intermediate, alpha-ketoglutate. Alpha-ketoglutarate is important for driving the malate-aspartate shuttle and is one of the most important nitrogen transporters. In addition, alpha-ketoglutarate is a precursor to the neurotransmitters glutamate and GABA. – Arginine AKG is a good form of Arginine proven to help build strength and give pumps.
Arginine Orotate: Arginine bound to orotic acid results in a longer duration of action and improved absorption of arginine. – Another form of Arginine that lasts longer in the body.Mormordica: Momordica is an herbal ingredient that has been used in China for over a thousand years. Momordica upregulates the expression of GLUT-4, the primary glucose transporter in skeletal muscle. This results in increased glucose and protein transport into muscle. This increased flow of nutrients also loads creatine and water into the muscle (8,9). – This stuff is really cool, it shuttles all the other stuff into the muscle. Unlike inferior products (like ALA) it pushes both amino acids and sugar into the muscle (all the above stuff are amino acids, which is why Cold Fusion works better than anything else).
Pomegranate Powder: Pomegranate juice contains potent antioxidants that protect nitric oxide (NO) from oxidative destruction. This results in an increase in the activity of NO as well as the duration of action (10). – Pomegranate is good for you in many ways, but it is shown to increase your pumps by extending the life of NO.
Spirulina: Spirulina has been shown to decrease oxidative damage in skeletal muscle but it also has another interesting effect. Spirulina augments the vasodilating effect of nitric oxide while at the same time, decreasing vasoconstriction by reducing the synthesis and release of vasoconstricting eicosanoids (11). – Spirulina is known as an energy food that increases pumps and reduces muscular fatigue.
This unique combination of ingredients will give you the biggest pumps during your workouts while at the same time producing the optimal anabolic state. Hopefully, our dual explanations give you science guys and normal dudes a chance to see why Cold Fusion EX is so great. Oh, you have to taste it! It is freaking amazing.
ReferencesWu J, Wu Q, Huang J, Chen R, Cai M, Tan J. Effects of L-malate on physical stamina and activities of enzymes related to the malate-aspartate shuttle in liver of mice. Physiol Res. 2006 Mar 23Kingston R, Kearns S, Kelly C, Murray P. Effects of systemic and regional taurine on skeletal muscle function following ischaemia-reperfusion injury. J Orthop Res. Mar;23(2):310-4, 2005Bakker AJ, Berg HM. Effect of taurine on sarcoplasmic reticulum function and force in skinned fast-twitch skeletal muscle fibres of the rat. J Physiol. Jan 1;538(Pt 1):185-94, 2002Coburn JW, Housh DJ, Housh TJ, Malek MH, Beck TW, Cramer JT, Johnson GO, Donlin PE. Effects of leucine and whey protein supplementation during eight weeks of unilateral resistance training. J Strength Cond Res. May;20(2):284-91, 2006Hoffman J, Ratamess N, Kang J, Mangine G, Faigenbaum A, Stout J. Effect of creatine and beta-alanine supplementation on performance and endocrine responses in strength/power athletes. Int J Sport Nutr Exerc Metab. Aug;16(4):430-46, 2006Marquezi ML, Roschel HA, dos Santa Costa A, Sawada LA, Lancha AH Jr. Effect of aspartate and asparagine supplementation on fatigue determinants in intense exercise. Int J Sport Nutr Exerc Metab. Mar;13(1):65-75, 2003Lancha AH Jr, Recco MB, Abdalla DS, Curi R. Effect of aspartate, asparagine, and carnitine supplementation in the diet on metabolism of skeletal muscle during a moderate exercise. Physiol Behav. Feb;57(2):367-71, 1995Cummings E, Hundal HS, Wackerhage H, Hope M, Belle M, Adeghate E, Singh J. Momordica charantia fruit juice stimulates glucose and amino acid uptakes in L6 myotubes. Mol Cell Biochem. Jun;261(1-2):99-104, 2004McCarty MF. Does bitter melon contain an activator of AMP-activated kinase? Med Hypotheses. 63(2):340-3, 2004Ignarro LJ, Byrns RE, Sumi D, de Nigris F, Napoli C. Pomegranate juice protects nitric oxide against oxidative destruction and enhances the biological actions of nitric oxide. Nitric Oxide. Sep;15(2):93-102, 2006Paredes-Carbajal MC, Torres-Duran PV, Diaz-Zagoya JC, Mascher D, Juarez-Oropeza MA. Effects of dietary Spirulina maxima on endothelium dependent vasomotor responses of rat aortic rings. Life Sci. 61(15):PL 211-9, 1997
Formadrol Extreme- Boost Your Testosterone Levels
FORMADROL EXTREME - BUY NOW AT www.lgsciences.comBOOST YOUR TESTOSTERONE AND PROTECT IT!
Do you see the guys with MAN BOOBS? One of the unpleasant side effects of a really effective Testosterone cycle can be bloat, fat and “gyno”…as your Testosterone converts to Estrogen. Everybody wants the androgenic effects of Testosterone but who wants man boobs?So how do you increase your Testosterone and protect it from Estrogen conversion? Your Testosterone levels are always under attack – by age, a prohormone or steroid cycle, medications, many thing. Some of these even shut your production down completely. The last thing you need is your dwindling Testosterone turning into Estrogen. Everyone knows Estrogen can totally destroy every gain you get from your reps and all the good your good diet does you.Every time you try to add more Testosterone with injections, legal steroids, or natural testosterone boosters –your body tries to regain its hormonal balance. It converts your Testosterone into Estrogen.
So now, instead of being primed to build muscle and strength and feeling horny as a goat, you have Estrogen running around building fat, making you lose your hair and turning your motor off. Sometimes, your pituitary gets really confused and TURNS OFF Testosterone production COMPLETELY!
PROTECT YOUR TESTOSTERONEFORMADROL EXTREME™ ups your Testosterone levels, blocks Estrogen conversion and helps you create your own Testosterone, naturally. ,It’s the most powerful Anti-Estrogen, Pro-Testosterone Complex we could find. FORMADROL EXTREME™ combines two ferocious anti-estrogen ingredients absolutely obliterate it while increasing Testosterone at the same time.We combine a suicide inhibitor that attaches itself to the Aromatase enzyme. That’s the enzyme that tells your body to convert Testosterone to Estrogen and even worse, STOP producing Testosterone. And you know what kind of effect that will have – less hard body all the way around, less libido.
So we start by destroying that enzyme which blocks Estrogen conversion, which blocks water weight, fat gains and other negative effects. So far so good. The second ingredient blocks Estrogen too AND increases Testosterone. First, it reduces the production of ANY and ALL estrogen in the body causes you to SIGNIFICANTLY increase Testosterone production, naturally. Some studies show Testosterone Levels up 500%!
This same dynamite ingredient normalize your T-levels – which is particularly important if you’ve just finished a steroid or pro-hormone cycle that jacked up your Testosterone level. Without this, your body could SHUT DOWN TESTOSTERONE PRODUCTION!
You don’t have to worry about TESTOSTERONE shut down with FORMADROL EXTREME™. It skyrockets your TESTOSTERONE naturally and safely. Clinical studies with one of the main ingredients in FORMADROL EXTREME™ showed increases over 500% higher bioavailable testosterone than any other Testosterone booster available. And it did it in record time – in less than 2 weeks! If that wasn’t enough, Estradiol was slashed more than 50% on average in ALL subjects in the first week alone.
And as a bonus, studies show, one of our ingredients may help increase IGF-1 levels, which is an anabolic coveted in the very hard-core anabolic circles.
FORMADROL EXTREME - BUY NOW AT www.lgsciences.comBOOST YOUR TESTOSTERONE AND PROTECT IT!
Do you see the guys with MAN BOOBS? One of the unpleasant side effects of a really effective Testosterone cycle can be bloat, fat and “gyno”…as your Testosterone converts to Estrogen. Everybody wants the androgenic effects of Testosterone but who wants man boobs?So how do you increase your Testosterone and protect it from Estrogen conversion? Your Testosterone levels are always under attack – by age, a prohormone or steroid cycle, medications, many thing. Some of these even shut your production down completely. The last thing you need is your dwindling Testosterone turning into Estrogen. Everyone knows Estrogen can totally destroy every gain you get from your reps and all the good your good diet does you.Every time you try to add more Testosterone with injections, legal steroids, or natural testosterone boosters –your body tries to regain its hormonal balance. It converts your Testosterone into Estrogen.
So now, instead of being primed to build muscle and strength and feeling horny as a goat, you have Estrogen running around building fat, making you lose your hair and turning your motor off. Sometimes, your pituitary gets really confused and TURNS OFF Testosterone production COMPLETELY!
PROTECT YOUR TESTOSTERONEFORMADROL EXTREME™ ups your Testosterone levels, blocks Estrogen conversion and helps you create your own Testosterone, naturally. ,It’s the most powerful Anti-Estrogen, Pro-Testosterone Complex we could find. FORMADROL EXTREME™ combines two ferocious anti-estrogen ingredients absolutely obliterate it while increasing Testosterone at the same time.We combine a suicide inhibitor that attaches itself to the Aromatase enzyme. That’s the enzyme that tells your body to convert Testosterone to Estrogen and even worse, STOP producing Testosterone. And you know what kind of effect that will have – less hard body all the way around, less libido.
So we start by destroying that enzyme which blocks Estrogen conversion, which blocks water weight, fat gains and other negative effects. So far so good. The second ingredient blocks Estrogen too AND increases Testosterone. First, it reduces the production of ANY and ALL estrogen in the body causes you to SIGNIFICANTLY increase Testosterone production, naturally. Some studies show Testosterone Levels up 500%!
This same dynamite ingredient normalize your T-levels – which is particularly important if you’ve just finished a steroid or pro-hormone cycle that jacked up your Testosterone level. Without this, your body could SHUT DOWN TESTOSTERONE PRODUCTION!
You don’t have to worry about TESTOSTERONE shut down with FORMADROL EXTREME™. It skyrockets your TESTOSTERONE naturally and safely. Clinical studies with one of the main ingredients in FORMADROL EXTREME™ showed increases over 500% higher bioavailable testosterone than any other Testosterone booster available. And it did it in record time – in less than 2 weeks! If that wasn’t enough, Estradiol was slashed more than 50% on average in ALL subjects in the first week alone.
And as a bonus, studies show, one of our ingredients may help increase IGF-1 levels, which is an anabolic coveted in the very hard-core anabolic circles.
Liquid Masterdrol - Explosive Strength Prohormone
You may not know this but LegalGear’s Masterdrol Series was the best selling prohormone in our entire line, even better than Methyl 1-Test! We were the first to release methyl delivery systems, which revolutionized prosteroids and we have done it again with LIQUID MASTERDROL™! We give to you the final product in our MASTERDROL SERIES.The original Masterdrol was a huge success, quickly followed by Masterdrol V2 (RIP from the ban of 2004) and Methyl-Masterdrol (RIP in the FDA ban of 2005). We proudly give you Liquid Masterdrol, the final in the series…our ultimate creation. With the passing of LegalGear and the licensing of the product line to LG Sciences, we knew we needed to revive this monster and give the people what they want...the calls were too great, the people demanded the results…and of course we delivered AGAIN!Enter the discount code 01192 in the order notes section at checkout – with the Trifecta Stack and receive a free bottle of NO INFUSE, a free T-Shirt and a free Beanie with your order!www.lgsciences.comThe last of the TRUE prohormones!Methyl 1-D and Liquid Masterdrol are the last true prohormones on the market today. Unlike illegal steroids and harsh prosteroids like Superdrol ™ and Halodrol-50 ™, you won’t see 10lbs in one week. As good as it feels to gain 10lbs in 1 week, you will most likely lose 70% of that because it is mostly cellular water weight. While this feels good, it gets you no closer to your ultimate goal and has potential harsh side effects that all steroids share. Methyl 1-D and Liquid Masterdrol are the SANE alternatives to harsh prosteroids. One warning, if you have done a cycle of illegal steroids or harsh prosteroids like Superdrol ™ or Halodrol-50 ™, you won’t “feel” our stack because your receptors are not clean. If, however you have avoided prohormones because of the harsh side effects, but always wanted prohormone gains, these products are for you! Users are reporting 7lbs of lean muscle in about two weeks, which is still amazing, but better yet is growth that your body can handle and maintain. Sure, it’s not like a crazy pro-steroid cycle, but you don’t keep steroid growth permanently, so what you gain in on the cycle is easily lost.What is Methyl 1-D?Methyl 1-D is similar to the original “andro”, but much better! It is a prohormone that converts into testosterone at a very high rate. Unlike other products, Methyl 1-D doesn't just increase your natural production, it gives your body more raw materials to make testosterone. Since the release of “andro” we have learned how to stop the negative side effects of boosting your testosterone to such super high levels. The metabolism support complex blocks conversion to estrogen (prevents gyno), blocks conversion to DHT (prevents hairloss) and makes the product more potent at raising testosterone levels.What is Liquid Masterdrol?Liquid Masterdrol is another prohormone that is targeted more toward increasing definition and strength. Methyl 1-D is used more for bulking while Liquid Masterdrol is for cutting and definition. Combined they make a very powerful stack. Liquid Masterdrol causes increased focus and intensity in the gym unlike any other product on the market. Methyl 1-D converts into Testosterone in the body, where Liquid Masterdrol converts into the end hormone Stanolone which is an androgenic hormone that increases strength and burns fat. Liquid Masterdrol can not convert into estrogen so there are little side effects and its complex gives amazing pumps.What is Formadrol?Formadrol is a natural anti-estrogen which “tricks” your body into producing more testosterone on it's own. Although Formadrol will not raise your testosterone levels as high as Methyl 1-D, it is still a very potent muscle builder. Formadrol can also be used to boost your natural production of testosterone after a cycle of Methyl 1-D (see stack information) and help the customer keep his gains.
Trifecta Stack:Methyl 1-D - 6 Capsules Per Day - For 4 WeeksLiquid Masterdrol 16cc (2 servings) on workout days 30 before workouts
Post Cycle Use Formadrol Extreme – 4 Capsules per day – For 2 Weeks after the Methyl 1-D, Liquid Masterdrol cycle
You may not know this but LegalGear’s Masterdrol Series was the best selling prohormone in our entire line, even better than Methyl 1-Test! We were the first to release methyl delivery systems, which revolutionized prosteroids and we have done it again with LIQUID MASTERDROL™! We give to you the final product in our MASTERDROL SERIES.The original Masterdrol was a huge success, quickly followed by Masterdrol V2 (RIP from the ban of 2004) and Methyl-Masterdrol (RIP in the FDA ban of 2005). We proudly give you Liquid Masterdrol, the final in the series…our ultimate creation. With the passing of LegalGear and the licensing of the product line to LG Sciences, we knew we needed to revive this monster and give the people what they want...the calls were too great, the people demanded the results…and of course we delivered AGAIN!Enter the discount code 01192 in the order notes section at checkout – with the Trifecta Stack and receive a free bottle of NO INFUSE, a free T-Shirt and a free Beanie with your order!www.lgsciences.comThe last of the TRUE prohormones!Methyl 1-D and Liquid Masterdrol are the last true prohormones on the market today. Unlike illegal steroids and harsh prosteroids like Superdrol ™ and Halodrol-50 ™, you won’t see 10lbs in one week. As good as it feels to gain 10lbs in 1 week, you will most likely lose 70% of that because it is mostly cellular water weight. While this feels good, it gets you no closer to your ultimate goal and has potential harsh side effects that all steroids share. Methyl 1-D and Liquid Masterdrol are the SANE alternatives to harsh prosteroids. One warning, if you have done a cycle of illegal steroids or harsh prosteroids like Superdrol ™ or Halodrol-50 ™, you won’t “feel” our stack because your receptors are not clean. If, however you have avoided prohormones because of the harsh side effects, but always wanted prohormone gains, these products are for you! Users are reporting 7lbs of lean muscle in about two weeks, which is still amazing, but better yet is growth that your body can handle and maintain. Sure, it’s not like a crazy pro-steroid cycle, but you don’t keep steroid growth permanently, so what you gain in on the cycle is easily lost.What is Methyl 1-D?Methyl 1-D is similar to the original “andro”, but much better! It is a prohormone that converts into testosterone at a very high rate. Unlike other products, Methyl 1-D doesn't just increase your natural production, it gives your body more raw materials to make testosterone. Since the release of “andro” we have learned how to stop the negative side effects of boosting your testosterone to such super high levels. The metabolism support complex blocks conversion to estrogen (prevents gyno), blocks conversion to DHT (prevents hairloss) and makes the product more potent at raising testosterone levels.What is Liquid Masterdrol?Liquid Masterdrol is another prohormone that is targeted more toward increasing definition and strength. Methyl 1-D is used more for bulking while Liquid Masterdrol is for cutting and definition. Combined they make a very powerful stack. Liquid Masterdrol causes increased focus and intensity in the gym unlike any other product on the market. Methyl 1-D converts into Testosterone in the body, where Liquid Masterdrol converts into the end hormone Stanolone which is an androgenic hormone that increases strength and burns fat. Liquid Masterdrol can not convert into estrogen so there are little side effects and its complex gives amazing pumps.What is Formadrol?Formadrol is a natural anti-estrogen which “tricks” your body into producing more testosterone on it's own. Although Formadrol will not raise your testosterone levels as high as Methyl 1-D, it is still a very potent muscle builder. Formadrol can also be used to boost your natural production of testosterone after a cycle of Methyl 1-D (see stack information) and help the customer keep his gains.
Trifecta Stack:Methyl 1-D - 6 Capsules Per Day - For 4 WeeksLiquid Masterdrol 16cc (2 servings) on workout days 30 before workouts
Post Cycle Use Formadrol Extreme – 4 Capsules per day – For 2 Weeks after the Methyl 1-D, Liquid Masterdrol cycle
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